With and without oxygen: respiration and fermentation

Grade 9 Science Worksheet - Challenge Me

NameDate

Circle the best answer.

  1. Read this first

    Use this

    ATP card (sourced values, per 1 glucose). The cell has no oxygen at first: fermentation, 2 ATP in total. After oxygen is added, pyruvate enters the mitochondrion: aerobic respiration, about 30 ATP in total.

  2. A cell that can ferment or use aerobic respiration has been fermenting 1 glucose. Pia adds oxygen. Predict the ATP total per glucose, and why.

    • It rises from 2 to about 30 ATP, since pyruvate now enters the mitochondrion
    • It rises from 0 to about 30 ATP, since the cell made no ATP before
    • It stays at 2 ATP, since fermentation and aerobic respiration make the same total
    • It falls from 2 to 0 ATP, since oxygen would stop glycolysis
  3. “model, not to scale” A chemical blocks the step that turns NADH into NAD+ in a muscle cell without oxygen. Ren predicts what happens to glycolysis and its ATP, and why.

    • Glycolysis slows to a stop and ATP from glucose stops, because NAD+ is not replaced
    • Glycolysis continues unchanged, because just the mitochondrion is affected
    • Glycolysis continues, because glycolysis takes the oxygen it needs from the muscle cell
    • Glycolysis continues at 2 ATP per glucose, because built-up NADH acts as fuel
  4. ATP card (sourced values)

    pathATP in total per 1 glucose
    Aerobic respirationabout 30
    Fermentation2
  5. Tala's class models a muscle cell given 10 glucose. With oxygen it makes about 300 ATP. Oxygen is removed, so only glycolysis and fermentation remain. Predict the new total.

    • It falls from about 300 to 0 ATP, because ATP needs oxygen
    • It falls from about 300 to 0 ATP, because muscle cells cannot ferment like yeast
    • It stays near 300 ATP, because glycolysis makes most of the cell's ATP
    • It falls from about 300 to 20 ATP
  6. Use this

    Two cards. Mud core card (model data, 4 rows). Columns: depth (cm), oxygen (mg/L). 0, 6. 1, 2. 3, 0. 8, 0. Final electron acceptor card (sourced facts, 4 rows). Columns: final electron acceptor, becomes. Oxygen, water. Sulfate, hydrogen sulfide gas. Iron (ferric), iron (ferrous). Carbon dioxide, methane.

  7. Zee's class reads two cards about wetland mud. Microbes at 8 cm make ATP with an electron transport chain. Which statement fits both cards?

    • Oxygen reads 0, so those microbes ferment, which is the same as ending a chain with sulfate
    • Oxygen reads 0, so those microbes make no ATP and release nothing
    • Oxygen reads 6, so those microbes end the chain with oxygen and make water
    • Oxygen reads 0, so a chain there must end with another acceptor, such as sulfate
  8. Use this

    Two cards. ATP card (sourced values, 1 row). Columns: path, ATP in total per 1 glucose. Fermentation, 2. NAD+ card (sourced facts, 1 row). Columns: stage, NAD+ regenerated per 1 glucose. Fermentation, 2.

  9. A model muscle sample made 50 ATP in total by fermentation alone. Bo uses both cards to find how many glucose were used and how many NAD+ were regenerated.

    • 25 glucose and 0 NAD+
    • About 2 glucose and 4 NAD+
    • 25 glucose and 25 NAD+
    • 25 glucose and 50 NAD+
  10. Notebook lines (model data)

    linenotebook says
    1Glycolysis nets 2 ATP per glucose and does not use oxygen
    2Fermentation turns NADH back into NAD+ so glycolysis can go on
    3Lactic acid fermentation in muscle runs in the mitochondrion with oxygen
    4Aerobic respiration makes about 30 ATP per glucose
  11. Esme's notebook lists four lines about glucose breakdown. One line has a flaw. Which line has the flaw?

    • Line 4: aerobic respiration makes fewer ATP per glucose than fermentation does
    • Line 1: glycolysis nets 4 ATP per glucose, not 2
    • Line 2: fermentation uses up NAD+ rather than regenerating it
    • Line 3: lactic acid fermentation runs in the cytoplasm and uses no oxygen
  12. Use this

    Notebook lines (model data, 4 rows). Columns: line, notebook says. 1, Yeast cells in bread dough ferment sugar and release CO2 gas. 2, Bacteria in yogurt ferment milk sugar into lactic acid. 3, Muscle cells cannot ferment, so only yeast cells use fermentation. 4, Some microbes in wetland mud use sulfate instead of oxygen.

  13. Jun's notebook lists four lines about microbes and cells. One line has a flaw. Which line has the flaw?

    • Line 2: yogurt bacteria need oxygen to ferment the milk sugar
    • Line 1: yeast fermentation makes lactic acid, not a gas
    • Line 4: microbes in mud make no ATP because they lack oxygen
    • Line 3: muscle cells and some bacteria also ferment, so yeast are not the only ones
  14. Kira's class concludes that sugar makes yeast release gas. Read about only. Kira's class reads about the dough test and concludes that sugar makes yeast release gas. What is the flaw in the test?

    • Cup B needed oxygen added, since fermentation cannot run without it
    • Height is the wrong measure, since ATP counts are what show fermentation
    • Cup B should have had more yeast than Cup A to be fair
    • Sugar and temperature both differ, so sugar cannot be named as the cause
  15. ATP card (sourced values)

    pathATP in total per 1 glucose
    Aerobic respirationabout 30
    Fermentation2
  16. Luz's classmate says sprinting muscle cells switch to fermentation because it makes more ATP than aerobic respiration. Do you agree?

    • Agree: fermentation nets about 30 ATP per glucose, so it is the better choice
    • Disagree: without oxygen, muscle cells make no ATP
    • Disagree: fermentation makes about 2 ATP per glucose against about 30, but it keeps glycolysis going when oxygen is short
    • Disagree: muscle cells do not ferment; yeast cells do
  17. Final electron acceptor card (sourced facts)

    final electron acceptorbecomes
    Oxygenwater
    Sulfatehydrogen sulfide gas
    Iron (ferric)iron (ferrous)
    Carbon dioxidemethane
  18. Noah's classmate says mud microbes that end an electron transport chain with sulfate are fermenting. Do you agree?

    • Disagree: sulfate users make no ATP, so neither process applies
    • Agree: any process that runs without oxygen is fermentation
    • Disagree: they use the oxygen in sulfate for aerobic respiration
    • Disagree: they run an electron transport chain ending with sulfate, which is anaerobic respiration
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With and without oxygen: respiration and fermentation

Answer Key - Grade 9 Science Worksheet - Challenge Me

Circle the best answer.

  1. Read this first

    Use this

    ATP card (sourced values, per 1 glucose). The cell has no oxygen at first: fermentation, 2 ATP in total. After oxygen is added, pyruvate enters the mitochondrion: aerobic respiration, about 30 ATP in total.

  2. A cell that can ferment or use aerobic respiration has been fermenting 1 glucose. Pia adds oxygen. Predict the ATP total per glucose, and why.

    • It rises from 2 to about 30 ATP, since pyruvate now enters the mitochondrion
    • It rises from 0 to about 30 ATP, since the cell made no ATP before
    • It stays at 2 ATP, since fermentation and aerobic respiration make the same total
    • It falls from 2 to 0 ATP, since oxygen would stop glycolysis
  3. “model, not to scale” A chemical blocks the step that turns NADH into NAD+ in a muscle cell without oxygen. Ren predicts what happens to glycolysis and its ATP, and why.

    • Glycolysis slows to a stop and ATP from glucose stops, because NAD+ is not replaced
    • Glycolysis continues unchanged, because just the mitochondrion is affected
    • Glycolysis continues, because glycolysis takes the oxygen it needs from the muscle cell
    • Glycolysis continues at 2 ATP per glucose, because built-up NADH acts as fuel
  4. ATP card (sourced values)

    pathATP in total per 1 glucose
    Aerobic respirationabout 30
    Fermentation2
  5. Tala's class models a muscle cell given 10 glucose. With oxygen it makes about 300 ATP. Oxygen is removed, so only glycolysis and fermentation remain. Predict the new total.

    • It falls from about 300 to 0 ATP, because ATP needs oxygen
    • It falls from about 300 to 0 ATP, because muscle cells cannot ferment like yeast
    • It stays near 300 ATP, because glycolysis makes most of the cell's ATP
    • It falls from about 300 to 20 ATP
  6. Use this

    Two cards. Mud core card (model data, 4 rows). Columns: depth (cm), oxygen (mg/L). 0, 6. 1, 2. 3, 0. 8, 0. Final electron acceptor card (sourced facts, 4 rows). Columns: final electron acceptor, becomes. Oxygen, water. Sulfate, hydrogen sulfide gas. Iron (ferric), iron (ferrous). Carbon dioxide, methane.

  7. Zee's class reads two cards about wetland mud. Microbes at 8 cm make ATP with an electron transport chain. Which statement fits both cards?

    • Oxygen reads 0, so those microbes ferment, which is the same as ending a chain with sulfate
    • Oxygen reads 0, so those microbes make no ATP and release nothing
    • Oxygen reads 6, so those microbes end the chain with oxygen and make water
    • Oxygen reads 0, so a chain there must end with another acceptor, such as sulfate
  8. Use this

    Two cards. ATP card (sourced values, 1 row). Columns: path, ATP in total per 1 glucose. Fermentation, 2. NAD+ card (sourced facts, 1 row). Columns: stage, NAD+ regenerated per 1 glucose. Fermentation, 2.

  9. A model muscle sample made 50 ATP in total by fermentation alone. Bo uses both cards to find how many glucose were used and how many NAD+ were regenerated.

    • 25 glucose and 0 NAD+
    • About 2 glucose and 4 NAD+
    • 25 glucose and 25 NAD+
    • 25 glucose and 50 NAD+
  10. Notebook lines (model data)

    linenotebook says
    1Glycolysis nets 2 ATP per glucose and does not use oxygen
    2Fermentation turns NADH back into NAD+ so glycolysis can go on
    3Lactic acid fermentation in muscle runs in the mitochondrion with oxygen
    4Aerobic respiration makes about 30 ATP per glucose
  11. Esme's notebook lists four lines about glucose breakdown. One line has a flaw. Which line has the flaw?

    • Line 4: aerobic respiration makes fewer ATP per glucose than fermentation does
    • Line 1: glycolysis nets 4 ATP per glucose, not 2
    • Line 2: fermentation uses up NAD+ rather than regenerating it
    • Line 3: lactic acid fermentation runs in the cytoplasm and uses no oxygen
  12. Use this

    Notebook lines (model data, 4 rows). Columns: line, notebook says. 1, Yeast cells in bread dough ferment sugar and release CO2 gas. 2, Bacteria in yogurt ferment milk sugar into lactic acid. 3, Muscle cells cannot ferment, so only yeast cells use fermentation. 4, Some microbes in wetland mud use sulfate instead of oxygen.

  13. Jun's notebook lists four lines about microbes and cells. One line has a flaw. Which line has the flaw?

    • Line 2: yogurt bacteria need oxygen to ferment the milk sugar
    • Line 1: yeast fermentation makes lactic acid, not a gas
    • Line 4: microbes in mud make no ATP because they lack oxygen
    • Line 3: muscle cells and some bacteria also ferment, so yeast are not the only ones
  14. Kira's class concludes that sugar makes yeast release gas. Read about only. Kira's class reads about the dough test and concludes that sugar makes yeast release gas. What is the flaw in the test?

    • Cup B needed oxygen added, since fermentation cannot run without it
    • Height is the wrong measure, since ATP counts are what show fermentation
    • Cup B should have had more yeast than Cup A to be fair
    • Sugar and temperature both differ, so sugar cannot be named as the cause
  15. ATP card (sourced values)

    pathATP in total per 1 glucose
    Aerobic respirationabout 30
    Fermentation2
  16. Luz's classmate says sprinting muscle cells switch to fermentation because it makes more ATP than aerobic respiration. Do you agree?

    • Agree: fermentation nets about 30 ATP per glucose, so it is the better choice
    • Disagree: without oxygen, muscle cells make no ATP
    • Disagree: fermentation makes about 2 ATP per glucose against about 30, but it keeps glycolysis going when oxygen is short
    • Disagree: muscle cells do not ferment; yeast cells do
  17. Final electron acceptor card (sourced facts)

    final electron acceptorbecomes
    Oxygenwater
    Sulfatehydrogen sulfide gas
    Iron (ferric)iron (ferrous)
    Carbon dioxidemethane
  18. Noah's classmate says mud microbes that end an electron transport chain with sulfate are fermenting. Do you agree?

    • Disagree: sulfate users make no ATP, so neither process applies
    • Agree: any process that runs without oxygen is fermentation
    • Disagree: they use the oxygen in sulfate for aerobic respiration
    • Disagree: they run an electron transport chain ending with sulfate, which is anaerobic respiration