Two-trait crosses with a 4 by 4 square
Grade 9 Science Worksheet

Circle the best answer.
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Parent card (pea plant P, genotype Rryy). Seed shape gene: alleles R (round) and r (wrinkled). Seed color gene: alleles y (green) and y (green).
Ren crosses two pea plants. Plant P has genotype Rryy. Which choice lists all the allele combinations plant P can pass on, one allele for each gene?
- RY and ry, with each capital kept with a capital
- Ry and ry
- Rr and yy, the plant's own gene pairs
- Ry, ry, RY and rY, as for a plant with Yy
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Parent card (pea plant Q, genotype rrYy). Seed shape gene: alleles r (wrinkled) and r (wrinkled). Seed color gene: alleles Y (yellow) and y (green).
Jun crosses two pea plants. Plant Q has genotype rrYy. Which choice lists all the allele combinations plant Q can pass on, one allele for each gene?
- rY and ry
- rr and Yy, the plant's own gene pairs
- rY, ry, RY and Ry, as for a plant with Rr
- RY and ry, with each capital kept with a capital
Use this
Allele combinations card. Parent 1 (genotype RrYy) can pass on 4 combinations: RY, Ry, rY and ry. Parent 2 (genotype RrYY) can pass on 2 combinations: RY and rY.
Kira crosses a plant with RrYy and a plant with RrYY. How many boxes does the Punnett square have?
- 4 boxes: 2 across and 2 down
- 6 boxes: 4 plus 2
- 16 boxes: 4 across and 4 down
- 8 boxes: 4 across and 2 down
Luz reads the filled square for RrYy × RrYy. What is the ratio of round yellow : round green : wrinkled yellow : wrinkled green?
- 1 : 1 : 1 : 1
- 3 : 1
- 9 : 3 : 3 : 1
- 1 : 3 : 3 : 9
Milo reads the same filled square but looks at seed shape alone. What is the ratio of round seeds to wrinkled seeds?
Use the picture in question 4.
- 1 : 1
- 3 : 1
- 9 : 7
- 9 : 1
Noah crosses RrYY with rrYy. Using the 4 boxes, what is the ratio of round yellow seeds to wrinkled yellow seeds?
Punnett square, 2 by 2, for pea seed shape (R round, r wrinkled) and seed color (Y yellow, y green). Top parent passes on: RY, rY. Side parent passes on: rY, ry. Row 1 (side rY): RrYY, rrYY. Row 2 (side ry): RrYy, rrYy. Phenotypes of the 4 boxes: round yellow or wrinkled yellow. Punnett square, 2 by 2, for pea seed shape (R round, r wrinkled) and seed color (Y yellow, y green). Top parent passes on: RY, rY. Side parent passes on: rY, ry. Row 1 (side rY): RrYY, rrYY. Row 2 (side ry): RrYy, rrYy. Phenotypes of the 4 boxes: round yellow or wrinkled yellow. Punnett square, 2 by 2, for pea seed shape (R round, r wrinkled) and seed color (Y yellow, y green). Side parent passes on: rY, ry. Top parent passes on RY rY Row 1 (side rY) RrYY rrYY Row 2 (side ry) RrYy rrYy Phenotypes of the 4 boxes: round yellow or wrinkled yellow. - 9 : 3
- 3 : 1
- 1 : 1
- 1 : 3
Chance card for RrYy × RrYy
Seed shape and seed color sort independently. Chance of round seeds: 3/4. Chance of wrinkled seeds: 1/4. Chance of yellow seeds: 3/4. Chance of green seeds: 1/4. Table: One-trait chances; columns trait and chance; rows round, wrinkled, yellow, green.
Zee crosses RrYy with RrYy again. What is the chance that one offspring is wrinkled and green?
- 1/16
- 1/2
- 1/4, the chance for one trait
- 1/9, from the 9 in the ratio
Ari crosses RrYy with RrYy once more. What is the chance that one offspring is round and green?
- 3/16
- 1
- 3/4, the chance of round alone
- 9/16, the chance of round and yellow
Model data
160 seeds from the cross RrYy × RrYy. The 16 boxes of the square are equally likely, and the expected boxes are round yellow 9, round green 3, wrinkled yellow 3 and wrinkled green 1. Table: Model total of seeds; columns group and boxes of 16; rows seeds in the model total, round yellow, round green, wrinkled yellow, wrinkled green.
Esme reads a model total of 160 seeds from RrYy × RrYy. About how many seeds are expected to be round and green?
- 30 seeds
- 90 seeds
- 3 seeds
- 120 seeds
Model data
64 seeds from the cross RrYy × RrYy. The 16 boxes of the square are equally likely, and the expected boxes are round yellow 9, round green 3, wrinkled yellow 3 and wrinkled green 1. Table: Model total of seeds; columns group and boxes of 16; rows seeds in the model total, round yellow, round green, wrinkled yellow, wrinkled green.
Pia reads a model total of 64 seeds from RrYy × RrYy. About how many seeds are expected to be wrinkled and yellow?
- 3 seeds
- 36 seeds
- 12 seeds
- 48 seeds