Two-trait crosses with a 4 by 4 square
Grade 9 Science Worksheet - Building Confidence

Circle the best answer.
Read this first
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Parent card (pea plant, genotype RrYy). Seed shape gene: alleles R (round) and r (wrinkled). Seed color gene: alleles Y (yellow) and y (green). Table: Parent card; columns gene and alleles in the plant; rows seed shape, seed color.
Ari reads a parent card for one pea plant. Which choice lists all the allele combinations this plant can pass on, one allele for each gene?
- RY, Ry, rY and ry
- Rr and Yy, the plant's own gene pairs
- RY, Ry and rY
- RY and ry, with each capital kept with a capital
Use this
Parent card (pea plant, genotype RrYY). Seed shape gene: alleles R (round) and r (wrinkled). Seed color gene: alleles Y (yellow) and Y (yellow). Table: Parent card; columns gene and alleles in the plant; rows seed shape, seed color.
Bo reads a parent card for another pea plant. Which choice lists all the allele combinations this plant can pass on, one allele for each gene?
- RY and rY
- RY, Ry, rY and ry, as for a plant with Yy
- Rr and YY, the plant's own gene pairs
- RY and ry, with each capital kept with a capital
Use this
Parent card (pea plant, genotype RrYy). The seed shape gene and the seed color gene are on different chromosomes. The combination in question is Ry: R from the seed shape gene and y from the seed color gene. Table: Parent card; columns gene and alleles in the plant; rows seed shape, seed color.
The genes for seed shape and seed color are on different chromosomes. Esme asks why a plant with RrYy can pass on the combination Ry. What is the reason?
- R stays with Y, so RY and ry are the combinations passed on
- Ry appears because y is dominant over Y
- The two genes sort independently, so R can go with either Y or y
- The plant passes on Rr as one unit and Yy as one unit
Jun fills in the square for RrYy × RrYy. Which genotype goes in the blank box?
- RrYy (R: one capital, one small; Y: one capital, one small)
- RRyy (R: two capital; Y: two small)
- rryy (R: two small; Y: two small)
- Rryy (R: one capital, one small; Y: two small)
Kira fills in another box of the square for RrYy × RrYy. Which genotype goes in the blank box?
Punnett square, 4 by 4, for pea seed shape (R round, r wrinkled) and seed color (Y yellow, y green). Top parent passes on: RY, Ry, rY, ry. Side parent passes on: RY, Ry, rY, ry. Row 1 (side RY): RRYY, RRYy, RrYY, RrYy. Row 2 (side Ry): RRYy, RRyy, RrYy, Rryy. Row 3 (side rY): ?, RrYy, rrYY, rrYy. Row 4 (side ry): RrYy, Rryy, rrYy, rryy. Punnett square, 4 by 4, for pea seed shape (R round, r wrinkled) and seed color (Y yellow, y green). Top parent passes on: RY, Ry, rY, ry. Side parent passes on: RY, Ry, rY, ry. Row 1 (side RY): RRYY, RRYy, RrYY, RrYy. Row 2 (side Ry): RRYy, RRyy, RrYy, Rryy. Row 3 (side rY): ?, RrYy, rrYY, rrYy. Row 4 (side ry): RrYy, Rryy, rrYy, rryy. Punnett square, 4 by 4, for pea seed shape (R round, r wrinkled) and seed color (Y yellow, y green). Top parent passes on: RY, Ry, rY, ry. Side parent passes on: RY, Ry, rY, ry. RY Ry rY ry Row 1 (side RY) RRYY RRYy RrYY RrYy Row 2 (side Ry) RRYy RRyy RrYy Rryy Row 3 (side rY) ? RrYy rrYY rrYy Row 4 (side ry) RrYy Rryy rrYy rryy - RrYY (R: one capital, one small; Y: two capital)
- RrYy (R: one capital, one small; Y: one capital, one small)
- rrYY (R: two small; Y: two capital)
- RRYY (R: two capital; Y: two capital)
Use this
Trait card for pea seeds, letter and seed trait. R: round (dominant). r: wrinkled (recessive). Y: yellow (dominant). y: green (recessive). Plant genotype: RrYy.
Milo reads a trait card. What do the seeds of a plant with genotype RrYy look like?
- Wrinkled and yellow
- Wrinkled and green
- Round and green
- Round and yellow
Use this
Trait card for pea seeds, letter and seed trait. R: round (dominant). r: wrinkled (recessive). Y: yellow (dominant). y: green (recessive). Plant genotype: rrYy.
Noah reads the same trait card. What do the seeds of a plant with genotype rrYy look like?
- Wrinkled and green
- Round and green
- Round and yellow
- Wrinkled and yellow
Use this
Trait card for pea seeds, letter and seed trait. R: round (dominant). r: wrinkled (recessive). Y: yellow (dominant). y: green (recessive). Plant genotype: Rryy.
Pia reads the trait card once more. What do the seeds of a plant with genotype Rryy look like?
- Round and green
- Round and yellow
- Wrinkled and green
- Wrinkled and yellow
Punnett square, 4 by 4, for pea seed shape (R round, r wrinkled) and seed color (Y yellow, y green). Top parent passes on: RY, Ry, rY, ry. Side parent passes on: RY, Ry, rY, ry. Row 1 (side RY): RRYY, RRYy, RrYY, RrYy. Row 2 (side Ry): RRYy, RRyy, RrYy, Rryy. Row 3 (side rY): RrYY, RrYy, rrYY, rrYy. Row 4 (side ry): RrYy, Rryy, rrYy, rryy. Phenotypes of the 16 boxes: round yellow, round green, wrinkled yellow or wrinkled green. Punnett square, 4 by 4, for pea seed shape (R round, r wrinkled) and seed color (Y yellow, y green). Top parent passes on: RY, Ry, rY, ry. Side parent passes on: RY, Ry, rY, ry. Row 1 (side RY): RRYY, RRYy, RrYY, RrYy. Row 2 (side Ry): RRYy, RRyy, RrYy, Rryy. Row 3 (side rY): RrYY, RrYy, rrYY, rrYy. Row 4 (side ry): RrYy, Rryy, rrYy, rryy. Phenotypes of the 16 boxes: round yellow, round green, wrinkled yellow or wrinkled green. Punnett square, 4 by 4, for pea seed shape (R round, r wrinkled) and seed color (Y yellow, y green). Top parent passes on: RY Ry rY ry Side parent passes on: RY RRYY RRYy RrYY RrYy Side parent passes on: Ry RRYy RRyy RrYy Rryy Side parent passes on: rY RrYY RrYy rrYY rrYy Side parent passes on: ry RrYy Rryy rrYy rryy Row 1 (side RY); Row 2 (side Ry); Row 3 (side rY); Row 4 (side ry). Phenotypes of the 16 boxes: round yellow, round green, wrinkled yellow or wrinkled green. Tala reads the same filled square. How many of the 16 boxes show round, yellow seeds?
- 1 box
- 3 boxes
- 12 boxes
- 9 boxes
Zee reads the same filled square. How many of the 16 boxes show wrinkled, yellow seeds?
Use the picture in question 9.
- 1 box
- 9 boxes
- 3 boxes
- 4 boxes