The nitrogen cycle
Grade 9 Science Worksheet

Circle the best answer.
Read this first
Use the table
Card (model data). Garden plots at the end of one season.
plot crop nitrate at start (mg per kg) nitrate at end (mg per kg) 1 peas 10 16 2 grass 10 7 3 beans 12 19 4 grass 12 9 Tala reads the card. By how much did the nitrate in Plot 1 change from the start to the end?
- It fell by 6 mg per kg
- It rose by 16 mg per kg
- It rose by 6 mg per kg
- It rose by 26 mg per kg
Zee compares two plots on the card. At the end, how much more nitrate does Plot 1 have than Plot 2?
- 9 mg per kg
- 3 mg per kg
- 6 mg per kg
- 23 mg per kg
Bo reads the card. Which statement does the card support?
- Nitrate fell in the legume plots and rose in the grass plots
- The crop made no difference to the change in nitrate
- Nitrate rose most in Plot 4, which grew grass
- Nitrate rose in the legume plots and fell in the grass plots
Esme reads the card of ways N₂ gets fixed. Which fixer is not a living thing?
- Lightning
- Cyanobacteria, since they live in the water
- Rhizobium, since it needs a plant root to live in
- Azotobacter, since it lives apart from any plant
Ari follows one nitrogen atom. Remains of a pea plant reach the soil. What belongs in the blank box?
- Ammonium
- Nothing, since decomposers destroy the nitrogen
- N₂ gas
- Nitrate
Luz follows nitrogen that grass roots took up as nitrate. Which route in the diagram returns it to the soil?
Flow diagram (model, not to scale), soil and air. Boxes: N₂ gas in air; living things (organic nitrogen); wastes and remains; ammonium; nitrite; nitrate. Arrows: from N₂ gas in air to living things (organic nitrogen): process nitrogen fixation, by free-living bacteria; Rhizobium bacteria in legume root nodules; cyanobacteria. from living things (organic nitrogen) to wastes and remains: process wastes and remains pass to the soil. from wastes and remains to ammonium: process ammonification, by decomposer bacteria and fungi. from ammonium to nitrite: process nitrification, first step, by nitrifying bacteria such as Nitrosomonas. from nitrite to nitrate: process nitrification, second step, by similar nitrifying bacteria. from nitrate to living things (organic nitrogen): process uptake by plant roots. from nitrate to N₂ gas in air: process denitrification, by denitrifying bacteria such as Pseudomonas and Clostridium. Flow diagram (model, not to scale), soil and air. Boxes: N₂ gas in air; living things (organic nitrogen); wastes and remains; ammonium; nitrite; nitrate. Arrows: from N₂ gas in air to living things (organic nitrogen): process nitrogen fixation, by free-living bacteria; Rhizobium bacteria in legume root nodules; cyanobacteria. from living things (organic nitrogen) to wastes and remains: process wastes and remains pass to the soil. from wastes and remains to ammonium: process ammonification, by decomposer bacteria and fungi. from ammonium to nitrite: process nitrification, first step, by nitrifying bacteria such as Nitrosomonas. from nitrite to nitrate: process nitrification, second step, by similar nitrifying bacteria. from nitrate to living things (organic nitrogen): process uptake by plant roots. from nitrate to N₂ gas in air: process denitrification, by denitrifying bacteria such as Pseudomonas and Clostridium. process: nitrogen fixation by: free-living bacteria; Rhizobium bacteria in legume root nodules; cyanobacteria process: denitrification by: denitrifying bacteria such as Pseudomonas and Clostridium process: uptake by plant roots process: wastes and remains pass to the soil process: ammonification by: decomposer bacteria and fungi process: nitrification, first step by: nitrifying bacteria such as Nitrosomonas process: nitrification, second step by: similar nitrifying bacteria N₂ gas in air living things (organic nitrogen) wastes and remains ammonium nitrite nitrate model - Fixation, which reverses the uptake by roots
- No route, since nitrogen that plants take up is used up
- Wastes and remains, then ammonification
- Denitrification, which makes ammonium from nitrate
Esme follows an ammonium ion in the soil. What does it become next, and which bacteria change it?
Flow diagram (model, not to scale), soil and air. Boxes: ammonium; blank; nitrate. Arrows: from ammonium to blank: process nitrification, first step, by blank. from blank to nitrate: process nitrification, second step, by similar nitrifying bacteria. Flow diagram (model, not to scale), soil and air. Boxes: ammonium; blank; nitrate. Arrows: from ammonium to blank: process nitrification, first step, by blank. from blank to nitrate: process nitrification, second step, by similar nitrifying bacteria. process: nitrification, first step by: blank process: nitrification, second step by: similar nitrifying bacteria ammonium blank nitrate model - Nitrate, by nitrifying bacteria such as Nitrosomonas
- Nitrite, by denitrifying bacteria such as Pseudomonas
- N₂ gas, by denitrifying bacteria such as Pseudomonas
- Nitrite, by nitrifying bacteria such as Nitrosomonas
Farmers add ammonium nitrate fertilizer to soil. Milo asks which forms of nitrogen plant roots can take up from it.
- N₂ gas, which fills the air spaces in the soil
- Nitrate and ammonium
- Nitrite, which fixation makes from N₂ gas
- Wastes and remains, before decomposers change them
Use the table
Card (model data). Soil nitrate in one plot over one week.
item mg per kg nitrate at the start of the week 20 added by nitrification 9 removed by plant uptake 6 removed by denitrification 2 Noah reads a budget card for one plot. How much nitrate is in the soil at the end of the week?
- 21 mg per kg
- 37 mg per kg
- 25 mg per kg
- 23 mg per kg
Pia reads a card for Plot 2. Grass roots took up nitrate, and the nitrate in the soil fell. What can happen to that nitrogen later?
- It is used up, so it cannot return to the soil
- It moves into root nodules, where Rhizobium fix it
- It joins wastes and remains, then returns to the soil as ammonium
- It is destroyed by decomposers in the soil