How cell parts work together to make and ship a protein
Grade 9 Science Worksheet

Circle the best answer.
Cell cutaway (animal cell, model, not to scale). Ari's class studies a gland cell that releases a digestive enzyme, a protein. Letter C, the Golgi apparatus, stops working. What most likely happens to the enzyme?
- It is held up at the plasma membrane, because the Golgi comes last
- It is made and folded but not packaged, so it stays in the cell
- It still leaves the cell, because each part works alone
- No enzyme is made, because the Golgi apparatus builds the protein
Read this first
Use this
Cell cutaway (animal cell, model, not to scale). The route has 7 stations, numbered 1 to 7, joined by arrows. Letter A marks the large round body near the middle, with a double wall (station 1). Letter B marks a small gap in the wall of the large round body (station 2). Letter C marks the flat folded sacs studded with tiny dots, joined to the wall of the large round body (station 3). Letter D marks the stack of curved smooth sacs, away from the dotted sacs (station 5).
Bo studies a cell that releases a protein. Its nuclear pores, letter B, become blocked. What most likely happens at the rough ER, letter C?
- The nucleus builds the protein inside and passes it on, so release goes on
- The Golgi apparatus builds the protein instead, so release goes on
- Protein piles up at the Golgi, because the pore comes after the Golgi
- Little new protein is built, because mRNA cannot reach the ribosomes
Cell cutaway (animal cell, model, not to scale). Esme reads that vesicle C can no longer bud from the rough ER, letter B. Where does a new protein most likely pile up?
Cell cutaway (animal cell, model, not to scale). The route has 7 stations, numbered 1 to 7, joined by arrows. Letter A marks the large round body near the middle, with a double wall (station 1). Letter B marks the flat folded sacs studded with tiny dots, joined to the wall of the large round body (station 3). Letter C marks a small bubble between the dotted sacs and the stack of curved smooth sacs (station 4). Letter D marks the stack of curved smooth sacs, away from the dotted sacs (station 5). Cell cutaway (animal cell, model, not to scale). The route has 7 stations, numbered 1 to 7, joined by arrows. Letter A marks the large round body near the middle, with a double wall (station 1). Letter B marks the flat folded sacs studded with tiny dots, joined to the wall of the large round body (station 3). Letter C marks a small bubble between the dotted sacs and the stack of curved smooth sacs (station 4). Letter D marks the stack of curved smooth sacs, away from the dotted sacs (station 5). A 1 2 B 3 C 4 D 5 6 7 - In the Golgi anyway, because the Golgi works alone to collect it
- In the Golgi anyway, because a vesicle is a permanent pipe between them
- In the rough ER, because nothing carries it to the Golgi apparatus
- In the Golgi, because the Golgi builds a new copy of the protein
Use the table
Pulse-chase card, read about only: a teacher added labeled amino acids to a gland cell for a short time, then followed the label. Where the labeled enzyme is found (model data)
minutes after labeling most label found in 3 rough ER 12 transport vesicles 25 Golgi apparatus 40 secretory vesicles 60 outside the cell Jun reads a pulse-chase card. The label is in the Golgi apparatus at 25 minutes. Where is it found next, and why?
- In secretory vesicles, because the Golgi packages proteins for shipping
- In transport vesicles, which stay fixed beside the Golgi apparatus
- Back in the rough ER, because the Golgi sends proteins back to be built
- In the Golgi still, because the Golgi builds more protein from the label
Pulse-chase card, read about only: a teacher followed a labeled membrane protein through a cell. Kira reads a card for a membrane protein. At 45 minutes the label is in the plasma membrane. What carried it there?
Pulse-chase card, read about only: a teacher followed a labeled membrane protein through a cell. Where a labeled membrane protein is found (model data), 4 rows. Columns: minutes after labeling, most label found in. 5, rough ER; 15, Golgi apparatus; 30, secretory vesicles; 45, plasma membrane. Pulse-chase card, read about only: a teacher followed a labeled membrane protein through a cell. Where a labeled membrane protein is found (model data), 4 rows. Columns: minutes after labeling, most label found in. 5, rough ER; 15, Golgi apparatus; 30, secretory vesicles; 45, plasma membrane. Where a labeled membrane protein is found (model data) minutes after labeling most label found in 5 rough ER 15 Golgi apparatus 30 secretory vesicles 45 plasma membrane - A secretory vesicle from the Golgi that fused with the membrane
- A transport vesicle from the rough ER, skipping the Golgi apparatus
- The nucleus, which sent the finished protein straight there
- A permanent pipe running from the rough ER to the membrane
Parts counted in one slice of each cell (model data)
cell rough ER sacs mitochondria P 48 12 Q 9 85 Milo reads counts for two cells. One is from a salivary gland and releases a digestive enzyme. The other is a muscle cell. Which pairing do the counts support?
- Q is the gland cell, because cells that secrete proteins have many mitochondria
- Cannot tell, because each part works alone, so counts do not follow the job
- P is the gland cell, since secreting cells have abundant rough ER
- P is the gland cell, because mitochondria build the digestive enzyme
“Cell cutaway” Pia reads that a muscle cell has a very high concentration of one part that produces ATP. Which lettered part is it?
Cell cutaway (animal cell, model, not to scale). Letter A marks a bean-shaped part with a folded inside. Letter B marks the large round body near the middle, with a double wall. Letter C marks the flat folded sacs studded with tiny dots, joined to the wall of the large round body. Letter D marks the stack of curved smooth sacs, away from the dotted sacs. Cell cutaway (animal cell, model, not to scale). Letter A marks a bean-shaped part with a folded inside. Letter B marks the large round body near the middle, with a double wall. Letter C marks the flat folded sacs studded with tiny dots, joined to the wall of the large round body. Letter D marks the stack of curved smooth sacs, away from the dotted sacs. animal cell, model, not to scale A B C D - Letter C, the rough ER, which produces ATP on its ribosomes
- Letter D, the Golgi apparatus, which packages the proteins muscle cells use
- Letter B, the nucleus, which builds the proteins that make muscle contract
- Letter A, the mitochondrion, which produces the ATP the muscle cell uses
Ren asks how the rough ER (A) and the Golgi apparatus (C) work together on a protein that leaves the cell. Which statement is right?
Cell cutaway (animal cell, model, not to scale). The route has 7 stations, numbered 1 to 7, joined by arrows. Letter A marks the flat folded sacs studded with tiny dots, joined to the wall of the large round body (station 3). Letter B marks a small bubble between the dotted sacs and the stack of curved smooth sacs (station 4). Letter C marks the stack of curved smooth sacs, away from the dotted sacs (station 5). Letter D marks a small bubble between the stack of curved sacs and the cell edge (station 6). Cell cutaway (animal cell, model, not to scale). The route has 7 stations, numbered 1 to 7, joined by arrows. Letter A marks the flat folded sacs studded with tiny dots, joined to the wall of the large round body (station 3). Letter B marks a small bubble between the dotted sacs and the stack of curved smooth sacs (station 4). Letter C marks the stack of curved smooth sacs, away from the dotted sacs (station 5). Letter D marks a small bubble between the stack of curved sacs and the cell edge (station 6). 3 A 4 B 5 C 6 D 1 2 7 - Each part works alone: the Golgi builds its own second copy of the protein
- The Golgi builds the protein first, then the rough ER packages it for release
- The rough ER makes and folds it, then a vesicle hands it to the Golgi
- A permanent pipe joins the two parts, and the protein slides through it
Cell cutaway (plant cell, model, not to scale). Part A makes sugar by photosynthesis. Tala studies a plant cell. Part A makes sugar by photosynthesis. How does part B, the bean-shaped part, use that sugar?
Cell cutaway (plant cell, model, not to scale). Letter A marks a green oval part with stacked discs inside. Letter B marks a bean-shaped part with a folded inside. Letter C marks the large round body near the middle, with a double wall. Part A makes sugar by photosynthesis. Cell cutaway (plant cell, model, not to scale). Letter A marks a green oval part with stacked discs inside. Letter B marks a bean-shaped part with a folded inside. Letter C marks the large round body near the middle, with a double wall. Part A makes sugar by photosynthesis. A green B C - B makes the sugar that A uses for photosynthesis
- B makes its own sugar, so it does not use the sugar from A
- B packages the sugar into vesicles, as the Golgi apparatus does
- B uses the sugar in cellular respiration to provide ATP energy
Use this
Cell cutaway (animal cell, model, not to scale). The route has 7 stations, numbered 1 to 7, joined by arrows. Letter A marks the large round body near the middle, with a double wall (station 1). Letter B marks a small gap in the wall of the large round body (station 2). Letter C marks the flat folded sacs studded with tiny dots, joined to the wall of the large round body (station 3).
Zee asks what the ribosomes on the rough ER (C) need from the nucleus (A) before they can build a protein. What is it?
Cell cutaway (animal cell, model, not to scale). The route has 7 stations, numbered 1 to 7, joined by arrows. Letter A marks the large round body near the middle, with a double wall (station 1). Letter B marks a small gap in the wall of the large round body (station 2). Letter C marks the flat folded sacs studded with tiny dots, joined to the wall of the large round body (station 3). Cell cutaway (animal cell, model, not to scale). The route has 7 stations, numbered 1 to 7, joined by arrows. Letter A marks the large round body near the middle, with a double wall (station 1). Letter B marks a small gap in the wall of the large round body (station 2). Letter C marks the flat folded sacs studded with tiny dots, joined to the wall of the large round body (station 3). 4 5 6 7 1 2 3 A B C animal cell - mRNA, which leaves A through B with the message for the protein
- Nothing, since the rough ER builds proteins without any help from A
- A packaged protein sent over from the Golgi apparatus
- The finished protein, which A builds inside and passes out through B