Transcription: from DNA to messenger RNA
Grade 9 Science Worksheet - Building Confidence

Circle the best answer.
Model gene (not to scale). Ari reads a model of one gene being copied into mRNA. DNA strand W is the strand the mRNA pairs with. What is strand W called?
- The new strand, the strand RNA polymerase builds
- The template strand, the strand RNA polymerase reads
- The coding strand, the strand with the same letters as the mRNA
- The promoter, the strand where transcription starts
Bo reads a model of transcription. Enzyme X moves along the DNA and builds the mRNA. What is enzyme X?
Model of transcription (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: T A C G G T. mRNA built so far, 5′ end on the left and 3′ end on the right: A U G C. Enzyme X is at the end of the mRNA built so far, where it adds the next base. Model of transcription (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: T A C G G T. mRNA built so far, 5′ end on the left and 3′ end on the right: A U G C. Enzyme X is at the end of the mRNA built so far, where it adds the next base. Model of transcription (not to scale) DNA template strand 3' end T A C G G T 5' end mRNA built so far 5' end A U G C Enzyme X 3' end - RNA polymerase, the enzyme that builds mRNA on both DNA strands at once
- RNA polymerase, the enzyme that builds mRNA on one DNA strand
- A ribosome, the structure that joins amino acids
- DNA polymerase, the enzyme that builds a new DNA strand
Read this first
Use this
Model strands (not to scale).
Jun fills in the blank in the mRNA built from this template. Which base goes in the blank?
Model strands (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: C T A G C T. mRNA, 5′ end on the left and 3′ end on the right: G A ? C G A. The ? is base 3 of the mRNA. Model strands (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: C T A G C T. mRNA, 5′ end on the left and 3′ end on the right: G A ? C G A. The ? is base 3 of the mRNA. DNA template strand 3' end 5' end C T A G C T mRNA 5' end 3' end G A ? C G A base 3 - G, guanine
- U, uracil
- A, adenine
- T, thymine
Kira fills in the blank in the mRNA built from this template. Which base goes in the blank?
Model strands (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: A T G C T C. mRNA, 5′ end on the left and 3′ end on the right: U ? C G A G. The ? is base 2 of the mRNA. Model strands (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: A T G C T C. mRNA, 5′ end on the left and 3′ end on the right: U ? C G A G. The ? is base 2 of the mRNA. DNA template strand 3' end 5' end A T G C T C mRNA 5' end 3' end U ? C G A G base 2 - U, uracil
- G, guanine
- A, adenine
- T, thymine
Luz fills in the blank in the mRNA built from this template. Which base goes in the blank?
Model strands (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: T A C G G T. mRNA, 5′ end on the left and 3′ end on the right: A U G ? C A. The ? is base 4 of the mRNA. Model strands (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: T A C G G T. mRNA, 5′ end on the left and 3′ end on the right: A U G ? C A. The ? is base 4 of the mRNA. DNA template strand 3' end 5' end T A C G G T mRNA 5' end 3' end A U G ? C A base 4 - U, uracil
- A, adenine
- C, cytosine
- G, guanine
Milo compares two mRNAs built from the same template. Which case is correct, and why?
Model strands (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: A C C T G A. mRNA in case P, 5′ end on the left and 3′ end on the right: U G G A C U. mRNA in case Q, 5′ end on the left and 3′ end on the right: T G G A C T. Model strands (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: A C C T G A. mRNA in case P, 5′ end on the left and 3′ end on the right: U G G A C U. mRNA in case Q, 5′ end on the left and 3′ end on the right: T G G A C T. DNA template strand 3' end 5' end A C C T G A mRNA in case P 5' end 3' end U G G A C U mRNA in case Q 5' end 3' end T G G A C T - Case P, because RNA has U where DNA has T
- Case Q, because mRNA keeps T the way DNA does
- Case P, because the mRNA copies the template letters
- Case Q, because RNA is made from the same letters as DNA
Model gene (not to scale). Noah compares two cases at one gene. Which case is right, and why?
Model gene (not to scale). DNA strand 1, 5′ end on the left and 3′ end on the right: A T G C C A. DNA strand 2, 3′ end on the left and 5′ end on the right: T A C G G T. mRNA in case P, 5′ end on the left and 3′ end on the right: A U G C C A. Case P: RNA polymerase builds one mRNA, on strand 2. Case Q: RNA polymerase builds two mRNAs, one on each strand. Model gene (not to scale). DNA strand 1, 5′ end on the left and 3′ end on the right: A T G C C A. DNA strand 2, 3′ end on the left and 5′ end on the right: T A C G G T. mRNA in case P, 5′ end on the left and 3′ end on the right: A U G C C A. Case P: RNA polymerase builds one mRNA, on strand 2. Case Q: RNA polymerase builds two mRNAs, one on each strand. Case P Case Q DNA strand 1 DNA strand 2 5' end 3' end 3' end 5' end A T G C C A T A C G G T RNA polymerase mRNA 5' end 3' end A U G C C A DNA strand 1 DNA strand 2 RNA polymerase mRNA mRNA - Case P, because RNA polymerase needs no template
- Case P, because the mRNA is built on the coding strand
- Case P, because one strand is the template for a gene
- Case Q, because both strands of a gene are templates
Pia compares two cases in a cell with a nucleus. Which case is right, and why?
Model gene in a cell with a nucleus (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: T A C G G T. mRNA, 5′ end on the left and 3′ end on the right: A U G C C A. Case P: the mRNA is built in the nucleus, then leaves it. Case Q: the mRNA is built in the cytoplasm. Model gene in a cell with a nucleus (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: T A C G G T. mRNA, 5′ end on the left and 3′ end on the right: A U G C C A. Case P: the mRNA is built in the nucleus, then leaves it. Case Q: the mRNA is built in the cytoplasm. cell nucleus cytoplasm DNA template strand 3' end 5' end T A C G G T Case P mRNA 5' end 3' end A U G C C A leaves nucleus Case Q mRNA 5' end A U G C C A 3' end - Case Q, because the ribosomes are in the cytoplasm
- Case P, because the mRNA stays in the nucleus
- Case P, because the DNA stays in the nucleus
- Case Q, because the DNA moves out to the cytoplasm
Use this
Model strands (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: T A C G G T A C G. mRNA, 5′ end on the left and 3′ end on the right: A U G C C A U G C.
Ren counts the bases in the mRNA strand of this model. How many bases does the mRNA have?
- 3 bases
- 18 bases
- 9 bases
- 8 bases
Use this
Model strands (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: T A C G G T. mRNA, 5′ end on the left and 3′ end on the right: A U G C C A. The U is base 2 of the mRNA.
Tala reads a model. Which template base sits across from the U in the mRNA?
- T, thymine
- A, adenine
- C, cytosine
- U, uracil