Transcription: from DNA to messenger RNA
Grade 9 Science Worksheet - Building Confidence

Circle the best answer.
Model gene (not to scale). Ari reads a model of one gene being copied into mRNA. DNA strand W is the strand the mRNA pairs with. What is strand W called?
- The new strand, the strand RNA polymerase builds
- The promoter, the strand where transcription starts
- The template strand, the strand RNA polymerase reads
- The coding strand, the strand with the same letters as the mRNA
“not to scale” Esme reads a model of a gene in a cell with a nucleus. The mRNA is built inside compartment Z. What is Z?
Model gene in a cell with a nucleus (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: T A C G G T. mRNA, 5′ end on the left and 3′ end on the right: A U G C C A. The DNA and the mRNA being built are both inside compartment Z. Model gene in a cell with a nucleus (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: T A C G G T. mRNA, 5′ end on the left and 3′ end on the right: A U G C C A. The DNA and the mRNA being built are both inside compartment Z. compartment Z cell DNA template strand 3' end T A C G G T 5' end mRNA 5' end A U G C C A 3' end - A ribosome, the structure that builds proteins
- A nuclear pore, the opening in the nuclear envelope
- The cytoplasm, the compartment where the ribosomes are
- The nucleus, the compartment that holds the DNA
Read this first
Use this
Model strands (not to scale).
Kira fills in the blank in the mRNA built from this template. Which base goes in the blank?
Model strands (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: A T G C T C. mRNA, 5′ end on the left and 3′ end on the right: U ? C G A G. The ? is base 2 of the mRNA. Model strands (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: A T G C T C. mRNA, 5′ end on the left and 3′ end on the right: U ? C G A G. The ? is base 2 of the mRNA. DNA template strand 3' end 5' end A T G C T C mRNA 5' end 3' end U ? C G A G base 2 - T, thymine
- U, uracil
- A, adenine
- G, guanine
Luz fills in the blank in the mRNA built from this template. Which base goes in the blank?
Model strands (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: T A C G G T. mRNA, 5′ end on the left and 3′ end on the right: A U G ? C A. The ? is base 4 of the mRNA. Model strands (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: T A C G G T. mRNA, 5′ end on the left and 3′ end on the right: A U G ? C A. The ? is base 4 of the mRNA. DNA template strand 3' end 5' end T A C G G T mRNA 5' end 3' end A U G ? C A base 4 - G, guanine
- C, cytosine
- U, uracil
- A, adenine
Milo compares two mRNAs built from the same template. Which case is correct, and why?
Model strands (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: A C C T G A. mRNA in case P, 5′ end on the left and 3′ end on the right: U G G A C U. mRNA in case Q, 5′ end on the left and 3′ end on the right: T G G A C T. Model strands (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: A C C T G A. mRNA in case P, 5′ end on the left and 3′ end on the right: U G G A C U. mRNA in case Q, 5′ end on the left and 3′ end on the right: T G G A C T. DNA template strand 3' end 5' end A C C T G A mRNA in case P 5' end 3' end U G G A C U mRNA in case Q 5' end 3' end T G G A C T - Case P, because the mRNA copies the template letters
- Case Q, because RNA is made from the same letters as DNA
- Case P, because RNA has U where DNA has T
- Case Q, because mRNA keeps T the way DNA does
Model gene (not to scale). Noah compares two cases at one gene. Which case is right, and why?
Model gene (not to scale). DNA strand 1, 5′ end on the left and 3′ end on the right: A T G C C A. DNA strand 2, 3′ end on the left and 5′ end on the right: T A C G G T. mRNA in case P, 5′ end on the left and 3′ end on the right: A U G C C A. Case P: RNA polymerase builds one mRNA, on strand 2. Case Q: RNA polymerase builds two mRNAs, one on each strand. Model gene (not to scale). DNA strand 1, 5′ end on the left and 3′ end on the right: A T G C C A. DNA strand 2, 3′ end on the left and 5′ end on the right: T A C G G T. mRNA in case P, 5′ end on the left and 3′ end on the right: A U G C C A. Case P: RNA polymerase builds one mRNA, on strand 2. Case Q: RNA polymerase builds two mRNAs, one on each strand. Case P Case Q DNA strand 1 DNA strand 2 5' end 3' end 3' end 5' end A T G C C A T A C G G T RNA polymerase mRNA 5' end 3' end A U G C C A DNA strand 1 DNA strand 2 RNA polymerase mRNA mRNA - Case P, because RNA polymerase needs no template
- Case P, because the mRNA is built on the coding strand
- Case P, because one strand is the template for a gene
- Case Q, because both strands of a gene are templates
Pia compares two cases in a cell with a nucleus. Which case is right, and why?
Model gene in a cell with a nucleus (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: T A C G G T. mRNA, 5′ end on the left and 3′ end on the right: A U G C C A. Case P: the mRNA is built in the nucleus, then leaves it. Case Q: the mRNA is built in the cytoplasm. Model gene in a cell with a nucleus (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: T A C G G T. mRNA, 5′ end on the left and 3′ end on the right: A U G C C A. Case P: the mRNA is built in the nucleus, then leaves it. Case Q: the mRNA is built in the cytoplasm. cell nucleus cytoplasm DNA template strand 3' end 5' end T A C G G T Case P mRNA 5' end 3' end A U G C C A leaves nucleus Case Q mRNA 5' end A U G C C A 3' end - Case P, because the DNA stays in the nucleus
- Case Q, because the ribosomes are in the cytoplasm
- Case P, because the mRNA stays in the nucleus
- Case Q, because the DNA moves out to the cytoplasm
Use this
Model strands (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: T A C G G T A C G. mRNA, 5′ end on the left and 3′ end on the right: A U G C C A U G C.
Ren counts the bases in the mRNA strand of this model. How many bases does the mRNA have?
- 3 bases
- 18 bases
- 9 bases
- 8 bases
Use this
Model strands (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: T A C G G T. mRNA, 5′ end on the left and 3′ end on the right: A U G C C A. The U is base 2 of the mRNA.
Tala reads a model. Which template base sits across from the U in the mRNA?
- U, uracil
- C, cytosine
- A, adenine
- T, thymine
Use this
DNA and RNA card (model data), 3 rows. Columns: feature, DNA, RNA. sugar, deoxyribose, ribose. bases, A T C G, A U C G. strands, two, usually one.
Zee reads a card that compares DNA and RNA. Which base is found in RNA but not in DNA?
- U, uracil
- C, cytosine
- A, adenine
- T, thymine