Active transport: pumps and vesicles
Grade 9 Science Worksheet - Challenge Me

Circle the best answer.
Read this first
Use the table
Lab card (model data) and drawing, not to scale. The card gives counts for pump P in one cell.
Minutes ATP used Sodium ions moved out Potassium ions moved in 5 8 24 16 10 15 45 30 15 21 63 42 Drawing: letter P points to a protein that reaches all the way across the bilayer, with an ATP label beside it. The card is read about only: a teacher counted the ions, and no one handled anything.
Bo reads the card for pump P. If the pattern continues, how many ATP has P used when 90 sodium ions have moved out, and how many potassium ions have moved in?
- 30 ATP and 60 potassium ions
- 90 ATP and 60 potassium ions
- 30 ATP and 45 potassium ions
- 45 ATP and 135 potassium ions
The test was read about only: a teacher ran it, and no one handled the cell. Luz reads a teacher’s lab card about a cell treated so it cannot make ATP. Which conclusion does the card support?
- Pumping stopped without ATP, so the pumps must have run out of potassium ions
- Crossings through proteins stopped without ATP, because proteins use ATP
- Oxygen crossing stopped without ATP, but pumping continued
- Pumping stopped without ATP, but crossings that need no ATP continued
The test was read about only: a teacher ran it, and no one handled the plant. Noah reads the card about a root hair cell. Why did the normal cell take in potassium ions even though it already held far more than the soil water?
- Channel proteins let potassium ions flow in, powered by ATP
- It used ATP to pump potassium ions toward the side with more
- Pumps moved potassium ions from where there are more to where there are fewer
- Pumps moved potassium ions in using the concentration difference
Use this
Lab card (model data) and drawing, not to scale. ATP was blocked at minute 0 in a cell. Table with 3 rows. Columns: Minutes after ATP was blocked | Sodium ions outside | Sodium ions inside | Glucose molecules moved in. Rows: 0 | 60 | 10 | 20; 20 | 40 | 30 | 8; 40 | 35 | 35 | 0. Drawing: letter G points to a carrier protein across the bilayer that carries sodium ions and glucose molecules into the cell together; no ATP label is beside G. The glucose count is for 1 minute. The test was read about only: a teacher ran it, and no one handled the cell.
Kira sees glucose uptake through G fall, even though G has no ATP label. Which reason fits the card?
- G needs no energy source, so the drop is a counting error
- G runs on the sodium gradient, and the gradient faded without the pump
- G uses ATP directly, so it stopped when ATP was blocked
- Glucose moved down its gradient, which flattened as more glucose built up
The test was read about only: a teacher ran it, and no one handled the cell. Ari sees the vesicle rows fall to 0 but the oxygen row stay at 18. Which reason explains the odd pattern?
- Vesicle uptake takes energy, but oxygen crosses down its gradient with none
- The particles came in by exocytosis, which stopped without ATP
- Oxygen crossing needs ATP too, so the 18 must be an error
- Vesicles form on their own, so the 0 rows must be errors
Use this
Membrane drawing (model, not to scale). A bilayer: a top row of round heads, tails meeting in the middle, and a bottom row of round heads, with watery fluid outside the cell above and watery fluid inside the cell below. Letter P points to a sodium-potassium pump with an ATP label beside it. Arrows show 3 sodium ions moving out and 2 potassium ions moving in, and 1 ATP being used in the cycle.
Esme’s notes say the sodium-potassium pump moves 3 sodium ions out and 2 potassium ions in, and needs no ATP. Which correction fixes the notes?
- It does move 3 sodium ions out and 2 potassium ions in, but each cycle uses ATP
- It moves 2 sodium ions out and 3 potassium ions in, and each cycle uses ATP
- It moves 3 sodium ions in and 2 potassium ions out, and each cycle uses ATP
- It moves 3 sodium ions out and 2 potassium ions in, and needs ATP on steep gradients
Use this
Membrane drawing (model, not to scale). A bilayer: a top row of round heads, tails meeting in the middle, and a bottom row of round heads, with watery fluid outside the cell above and watery fluid inside the cell below. Letter P points to a pump with an ATP label beside it. Letter C points to a channel protein with a tunnel and no ATP label. Outside the cell: 10 sodium ions. Inside the cell: 2 sodium ions.
Ren’s notes say every protein in a membrane uses ATP, so a cell that cannot make ATP cannot move any ion. Which correction fixes the notes?
- Channels use ATP to move ions, and pumps use the gradient
- Pumps use ATP to move ions down a gradient, and channels move ions up it
- Pumps and channels both use ATP, but pumps use more of it
- Pumps use ATP, but channels let ions flow down their gradient with no ATP
Membrane drawing (model, not to scale). Tala must pick the best claim, with its reason, about pump P in a root hair cell and the potassium ions near it.
- P can add potassium ions to the cell, because membrane proteins are powered by ATP
- P can add potassium ions to the cell, because ions move from the side with more to the side with fewer
- P can add potassium ions to the cell, because ATP lets it move them toward the side with more
- P can add potassium ions to the cell, because the concentration difference supplies the energy
Membrane drawing (model, not to scale). A gland cell's Golgi apparatus packaged an enzyme into a secretory vesicle. Milo must pick the best claim, with its reason, about the 12 enzyme molecules in vesicle V of a gland cell.
Membrane drawing (model, not to scale). A bilayer: a top row of round heads, tails meeting in the middle, and a bottom row of round heads, with watery fluid outside the cell above and watery fluid inside the cell below. A gland cell’s Golgi apparatus packaged an enzyme into a secretory vesicle. Letter V points to the vesicle, which touches the inside of the bottom row and holds 12 enzyme molecules. Outside the cell: 0 enzyme molecules. Membrane drawing (model, not to scale). A bilayer: a top row of round heads, tails meeting in the middle, and a bottom row of round heads, with watery fluid outside the cell above and watery fluid inside the cell below. A gland cell’s Golgi apparatus packaged an enzyme into a secretory vesicle. Letter V points to the vesicle, which touches the inside of the bottom row and holds 12 enzyme molecules. Outside the cell: 0 enzyme molecules. watery fluid outside the cell 0 enzyme molecules watery fluid inside the cell V 12 enzyme molecules - They will leave by phagocytosis, because the vesicle engulfs a large particle and carries it out
- They will leave by exocytosis, because the vesicle fuses with the membrane and releases them
- They will leave by endocytosis, because the vesicle fuses with the membrane and releases them
- They will leave by exocytosis, because vesicles drift out with no energy needed
Membrane drawing (model, not to scale). Zee must pick the best claim, with its reason, about proteins P and G in the drawing.
Membrane drawing (model, not to scale). A bilayer: a top row of round heads, tails meeting in the middle, and a bottom row of round heads, with watery fluid outside the cell above and watery fluid inside the cell below. Letter P points to a pump with an ATP label beside it that moves sodium ions out of the cell. Letter G points to a protein with no ATP label that carries sodium ions and glucose molecules into the cell together. Outside the cell: 60 sodium ions and 8 glucose molecules. Inside the cell: 10 sodium ions and 20 glucose molecules. Membrane drawing (model, not to scale). A bilayer: a top row of round heads, tails meeting in the middle, and a bottom row of round heads, with watery fluid outside the cell above and watery fluid inside the cell below. Letter P points to a pump with an ATP label beside it that moves sodium ions out of the cell. Letter G points to a protein with no ATP label that carries sodium ions and glucose molecules into the cell together. Outside the cell: 60 sodium ions and 8 glucose molecules. Inside the cell: 10 sodium ions and 20 glucose molecules. 60 sodium ions 8 glucose molecules watery fluid outside the cell P ATP G watery fluid inside the cell 10 sodium ions 20 glucose molecules - P is primary active transport and G is secondary, because G runs on the gradient P builds
- P and G are both primary active transport, because both use ATP directly
- P and G are both secondary active transport, because neither needs energy
- G is primary active transport and P is secondary, because G uses the ATP that P splits