Diffusion and osmosis
Grade 9 Science Worksheet - Challenge Me

Circle the best answer.
Ari reads a graph of particles on two sides of a membrane they cross. What does the flat part of both lines, after 30 minutes, show?
- The sides are equal near 50, with particles still crossing both ways
- Particles crossed from side 1 to side 2, and no particle crossed back
- The membrane ran out of energy for moving particles at 30 minutes
- Particles stopped crossing once the sides reached 50
Bo reads a graph of one substance crossing a membrane in warm water and in cold water. Which conclusion does the graph support?
Graph (model data). Particles that crossed a membrane, in warm and in cold water. 100 particles started on one side. Time (minutes) across, 0 to 40, a mark every 10. Particles crossed (count) up, 0 to 60, a mark every 10. Warm line joins (0, 0), (10, 30), (20, 45), (30, 50), (40, 50) with straight pieces. Cold line joins (0, 0), (10, 15), (20, 28), (30, 40), (40, 50) with straight pieces. Graph (model data). Particles that crossed a membrane, in warm and in cold water. 100 particles started on one side. Time (minutes) across, 0 to 40, a mark every 10. Particles crossed (count) up, 0 to 60, a mark every 10. Warm line joins (0, 0), (10, 30), (20, 45), (30, 50), (40, 50) with straight pieces. Cold line joins (0, 0), (10, 15), (20, 28), (30, 40), (40, 50) with straight pieces. Particles that crossed a membrane 0 10 20 30 40 0 10 20 30 40 50 60 Time (minutes) Particles crossed (count) Warm Cold 100 particles started on one side. - Warmth changes the end point: warm water ends higher than cold
- Cold water is slower because the membrane spends less energy there
- Warm particles stop moving after 30 minutes, when the line goes flat
- Warmth raises the rate but not the end point: both reach 50
Esme reads a graph of one substance moving into a lab chamber. The line climbs steeply, then flattens. Why does the climb slow down?
Graph (model data). Concentration in a lab chamber that a substance crosses into from a second chamber of the same size, which started at 80. Time (minutes) across, 0 to 30, a mark every 5. Concentration (units) up, 0 to 40, a mark every 10. The line joins (0, 0), (5, 20), (10, 30), (15, 35), (20, 38), (25, 39), (30, 40) with straight pieces. Graph (model data). Concentration in a lab chamber that a substance crosses into from a second chamber of the same size, which started at 80. Time (minutes) across, 0 to 30, a mark every 5. Concentration (units) up, 0 to 40, a mark every 10. The line joins (0, 0), (5, 20), (10, 30), (15, 35), (20, 38), (25, 39), (30, 40) with straight pieces. Graph (model data). Concentration in a lab chamber that a substance crosses into from a second chamber of the same size, which started at 80. 0 5 10 15 20 25 30 0 10 20 30 40 Time (minutes) Concentration (units) - The gradient shrinks as the chambers near equal, so diffusion slows
- The chamber is running low on energy to power diffusion
- Particles slow down and stop moving as the line flattens
- Particles cross into the chamber and do not cross back, so the first empties
Read this first
Use this
Lab card (model data). A U-tube had a membrane across the bottom that passes water but not sugar. The left arm held 3 sugar dots and the right arm held 9 at the start, and both levels began at 20 mL. Table with 3 rows. Columns: Time (minutes) | Left level (mL) | Right level (mL). Rows: 0 | 20 | 20; 10 | 17 | 23; 20 | 15 | 25. A teacher ran the test; read about only.
Kira reads a card for a U-tube with a membrane that passes water but not sugar. Which conclusion does the card support?
- Water moved right to left, toward less sugar, as the right level rose
- Water moved left to right, toward more sugar, as the left level fell 3 mL by 20 minutes
- Water moved left to right, toward more sugar, as the left level fell 5 mL by 20 minutes
- Sugar dots crossed to the right arm and raised its level
Use this
Lab card (model data). A lab-made bilayer held 10 carrier proteins for glucose. The card gives the glucose outside and the rate of crossing. Table with 4 rows. Columns: Glucose outside (units) | Rate (molecules per second). Rows: 2 | 5; 4 | 9; 8 | 12; 16 | 12. A teacher ran the test; read about only.
Luz reads a card about glucose crossing through carrier proteins. The rate stays at 12 when glucose outside rises from 8 to 16. Why?
- Glucose crossed the bilayer alone at 16 units, so carriers were not needed
- The carriers ran out of cell energy once glucose reached 8 units
- Glucose stopped moving at 8 units, because the sides reached equilibrium
- Every carrier was busy, so more glucose could not raise the rate
Use this
Notes card written by a student. Line 1: Diffusion moves a substance from higher to lower concentration. Line 2: The cell spends ATP to run diffusion. Line 3: Warmer conditions speed diffusion up.
Milo's notes on diffusion have three lines. One line is wrong. Which correction fixes the notes?
- Line 3 is wrong: warmer conditions slow diffusion down
- Line 2 is right: diffusion through proteins also spends ATP
- Line 1 is wrong: substances move from lower to higher concentration
- Line 2 is wrong: diffusion is passive, so the cell spends no ATP
Use this
Notes card written by a student. The left arm holds 2 sugar dots and the right arm holds 8. Line 1: Sugar cannot cross the membrane. Line 2: Water crosses, so water moves toward the left arm, where there is less sugar. Line 3: The left level will rise.
Noah's notes explain a U-tube with a membrane that passes water but not sugar. One line is wrong. Which correction fixes the notes?
- Water moves to the right arm, which has more sugar, so its level rises
- Water moves to the left arm, which has less sugar, so its level rises
- Sugar moves to the left arm, which has less sugar, so its level rises
- Water moves to the right arm, which has more sugar, so its level falls
Pia's notes say a molecule in diffusion only moves from high to low concentration, so it never moves back. Which correction fixes the notes?
Notes card and membrane drawing (model, not to scale). A bilayer of phospholipids with watery fluid outside the cell above and watery fluid inside the cell below. No proteins are drawn. Outside the cell: 12 oxygen molecules. Inside the cell: 4 oxygen molecules. A long arrow points inward and a short arrow points outward. Notes card and membrane drawing (model, not to scale). A bilayer of phospholipids with watery fluid outside the cell above and watery fluid inside the cell below. No proteins are drawn. Outside the cell: 12 oxygen molecules. Inside the cell: 4 oxygen molecules. A long arrow points inward and a short arrow points outward. Notes watery fluid outside the cell watery fluid inside the cell model, not to scale No proteins are drawn. 12 oxygen molecules 4 oxygen molecules O O O O O O O O O O O O O O O O inward outward - Molecules move both ways, but more move outward, so the net movement is outward
- Molecules move both ways until equilibrium, and then they stop moving
- Molecules move at random both ways; the net movement is from high to low
- No correction: molecules move from high to low, and a move back needs cell energy
Ren compares claims about glucose and carrier protein C when both sides hold 8 glucose molecules. Which claim, with its reason, fits?
Membrane drawing (model, not to scale). A bilayer of phospholipids with watery fluid outside the cell above and watery fluid inside the cell below. Letter C points to a protein that reaches all the way across the bilayer and changes shape to carry glucose. Outside the cell: 8 glucose molecules. Inside the cell: 8 glucose molecules. Membrane drawing (model, not to scale). A bilayer of phospholipids with watery fluid outside the cell above and watery fluid inside the cell below. Letter C points to a protein that reaches all the way across the bilayer and changes shape to carry glucose. Outside the cell: 8 glucose molecules. Inside the cell: 8 glucose molecules. model, not to scale watery fluid outside the cell watery fluid inside the cell glucose phospholipids C - Net movement inward, because the carrier points toward the inside
- No net movement of glucose, because the two sides match and the gradient is gone
- Net movement inward, because the carrier uses cell energy to pull glucose in
- No glucose crosses, because the carrier stops working when the sides match
Lab drawing (model, not to scale). Zee reads about a bag of lab membrane that passes water but not starch. Which claim about water, with its reason, fits?
Lab drawing (model, not to scale). A bag of lab membrane holds a starch solution with 8 starch dots. The bag sits in a beaker of plain water with no starch. The membrane passes water but not starch. The bag and the beaker hold the same volume of fluid. Lab drawing (model, not to scale). A bag of lab membrane holds a starch solution with 8 starch dots. The bag sits in a beaker of plain water with no starch. The membrane passes water but not starch. The bag and the beaker hold the same volume of fluid. beaker starch solution bag of lab membrane 8 starch dots no starch plain water passes water but not starch same volume of fluid - Water moves out of the bag, because it moves toward the side with less starch
- Water moves into the bag, because the beaker has more free water
- Water moves into the bag, because water molecules move in one direction
- Water moves into the bag, because the membrane spends energy to pull it in