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9th grade Diffusion and osmosis worksheet

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Diffusion and osmosis

Grade 9 Science Worksheet

NameDate

Circle the best answer.

  1. Read this first

    Use this

    Lab card (model data). Coloring particles in two chambers joined by a membrane they can cross. Table with 3 rows. Columns: Time (minutes) | Left chamber | Right chamber. Rows: 0 | 24 | 0; 5 | 16 | 8; 10 | 13 | 11. A teacher ran the test; read about only.

  2. Ari's class reads counts of food-coloring particles in two chambers joined by a membrane they can cross. What will the counts be after a long time?

    • About 12 in each chamber, with particles no longer crossing
    • About 12 in each chamber, though no particle crosses back to the left
    • About 24 in the right chamber, because particles end up on the far side
    • About 12 in each chamber, with particles still crossing both ways
  3. Lab membrane drawing (model, not to scale). Dots of one substance can cross the membrane. Bo watches a model: side 1 holds 18 dots and side 2 holds 6. A teacher adds 12 dots to side 2. What happens next?

    • The dots keep moving from side 1 to side 2 until side 2 holds more
    • The dots stop moving, since the two sides are equal
    • The dots move from side 2 to side 1, because side 2 was added last
    • There is no net movement, though the dots keep moving both ways
  4. No proteins are drawn. Esme reads a drawing with two gases near a cell membrane. Each gas crosses the bilayer alone. Which statement predicts the net movement?

    • Both gases move in, because the outside holds more molecules in total
    • Oxygen moves in, and the cell spends energy to move carbon dioxide out
    • Oxygen moves out and carbon dioxide moves in, because gases swap places
    • Oxygen moves in and carbon dioxide out, each down its own gradient
  5. Use this

    Lab card (model data). Time for food coloring to spread to the far side of a cup. Table with 2 rows. Columns: Water | Time to spread (seconds). Rows: warm water | 30; cold water | 90. The cups were the same size and held the same amount of water. A teacher ran the test; read about only.

  6. Jun's class drops food coloring into two cups of water and times how long the color takes to reach the far side. What does the card show?

    • The cups match, because diffusion is passive and needs no energy
    • Cold water spreads color faster, because dense water pushes particles along
    • Warmer water speeds diffusion, because particles have more thermal energy
    • Warmer water slows diffusion, because particles crowd together
  7. Use this

    Lab card (model data). Time for each substance to spread across a tank. Table with 2 rows. Columns: Substance | Molecule mass | Time to spread (seconds). Rows: L | lighter | 40; H | heavier | 100. Both tanks held the same warm water and the same starting gradient. A teacher ran the test; read about only.

  8. Luz's class reads a card with two substances in the same warm tank. A third substance, M, has molecules heavier than L's but lighter than H's. Predict M's time.

    • Under 40 seconds, since heavier molecules diffuse faster
    • Exactly 40 seconds, since the same gradient gives the same time
    • Exactly 140 seconds, since the two times add together
    • Between 40 and 100 seconds, since heavier molecules diffuse more slowly
  9. model, not to scale. Chloride ions cross a cell membrane through channel protein H, from 14 molecules outside to 5 inside. Milo classifies the transport. Which label fits?

    • Simple diffusion: passing between the tails, down the concentration gradient
    • Facilitated diffusion: passive, through a protein, down the gradient
    • Osmosis: chloride ions crossing toward the side with more water
    • Facilitated diffusion: through a protein, using energy from the cell
  10. Noah sorts four transport events shown on a membrane drawing. Which event is simple diffusion?

    • Oxygen slips between the tails, down its gradient, with no protein
    • Carbon dioxide slips between the tails, powered by cell energy
    • Glucose binds carrier M, which spends cell energy to change shape
    • Chloride ions pass through channel L, down their gradient
  11. Lab membrane drawing (model, not to scale). Pia reads a drawing of aquaporin channels in a lab membrane. Water crosses, but solute cannot. Which label fits the water movement?

    • Facilitated diffusion: the cell spends energy to open the aquaporin
    • Osmosis: movement of water that the cell powers with its energy
    • Osmosis: passive movement of water toward the side with less solute
    • Osmosis: passive water movement toward the side with more solute
  12. Use this

    Lab drawing (model, not to scale). A U-tube with a selectively permeable membrane across the bottom. Water crosses it; sugar dots cannot. The left arm holds 2 sugar dots and the right arm holds 7 sugar dots. Both arms hold the same volume of fluid at the start.

  13. Ren's class reads about a U-tube with a membrane at the bottom that passes water but not sugar. The arms start at the same level. Which arm's level rises?

    • The left arm, because water moves toward the side with less sugar
    • Neither arm, because water stops moving when the arms start level
    • The right arm, because water moves toward the side with more sugar
    • The right arm, because the sugar dots cross to the right
  14. Zee compares two U-tubes with a membrane that passes water but not sugar. Which tube shows the faster net movement of water, and why?

    • Tube 1, because its arms are closer to equal, so water moves faster
    • The tubes match, because water moves toward the right arm in both
    • Tube 2, because the membrane spends more energy on a bigger difference
    • Tube 2, because its arms differ more in sugar, so water moves faster
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9th grade Diffusion and osmosis worksheet – printable
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About this worksheet

Diffusion and osmosis. Every version has 10 questions, and the answer key prints on the second page.

What your child will learn

  • I can say what a concentration gradient is, and read which side of a drawing or table is more concentrated.
  • I can say that diffusion is the net movement of a substance from higher to lower concentration, that it is passive (the cell spends no energy), and that it goes on until there is no net movement.
  • I can say what dynamic equilibrium is: particles keep moving both ways, but there is no net change.
  • I can name the factors that change the rate (the size of the gradient, temperature, the mass of the particles, the density of the solvent, the distance) and predict which way each one pushes the rate.
  • I can tell simple diffusion (between the tails) from facilitated diffusion (through a channel or carrier protein), and say that both are passive and both go down the gradient.
  • I can say that osmosis is the diffusion of water across a selectively permeable membrane, toward the side with more solute (less free water), and predict which side of a U-tube gains water.

Standard: supports HS-LS1-3

Words to know

concentration: How much of a substance there is in a given space

concentration gradient: A difference in concentration between two places

diffusion: Net movement of a substance from higher to lower concentration

passive: Needs no energy from the cell

dynamic equilibrium: Particles keep moving, but there is no net movement

net movement: The overall change left over after movement in both directions

facilitated diffusion: Diffusion through a channel or carrier protein, still passive

channel protein: A protein with a tunnel for one kind of particle

Common mistakes

Your child thought particles stop moving once the amounts are equal. At equilibrium they keep moving both ways, but there is no net change. Ask what the dots are doing when the counts stay the same.

Your child thought the cell spends energy to make diffusion happen. Diffusion is passive: the particles' own motion down the gradient does the work. Ask who supplies the energy when a gas spreads out.

Your child had water moving toward the side with less dissolved solute. In osmosis water moves toward the side with more solute, where there is less free water. Ask which side has more free water and where the water goes.

Questions parents ask

What is the most common mistake with diffusion and osmosis?

Your child thought particles stop moving once the amounts are equal. At equilibrium they keep moving both ways, but there is no net change. Ask what the dots are doing when the counts stay the same.

Are these 9th grade science worksheets free to print?

Yes. All 6 printable versions at each level are free to print, with the answer key, and no account is needed.

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