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9th grade Cellular respiration: the equation and its three stages worksheet

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Cellular respiration: the equation and its three stages

Grade 9 Science Worksheet

NameDate

Circle the best answer.

  1. Read this first

    Equation card, from OpenStax Biology 2e

    C6H12O6 + 6O2 → 6CO2 + 6H2O + energy. Inputs in boxes on the left: glucose, C6H12O6; oxygen, 6O2. Outputs in boxes on the right: carbon dioxide, 6CO2; water, 6H2O. A wavy arrow points out: energy.

  2. Tala counts the oxygen atoms on each side of C6H12O6 + 6O2 → 6CO2 + 6H2O. How many are on the left side, and how many on the right side?

    • 18 on the left and 18 on the right
    • 12 on the left and 18 on the right
    • 12 on the left and 12 on the right
    • 18 on the left and 12 on the right
  3. Zee counts atoms in the respiration equation and finds both sides balanced. The right side also lists energy. What does the energy term show?

    • New energy that the cell creates during the reaction
    • Energy stored in the CO2 and water, kept for later
    • Energy moved out of glucose's bonds into ATP and heat
    • A product made of atoms that must also be counted
  4. Ari reads that a potato plant's cells break down 2 glucose in the dark. Using the equation, how many H2O form, and how many hydrogen atoms do they hold?

    • 0 H2O, since plant cells do not break glucose down
    • 6 H2O holding 12 hydrogen atoms
    • 12 H2O holding 12 hydrogen atoms
    • 12 H2O holding 24 hydrogen atoms
  5. Use this

    Flow diagram (model, not to scale), drawn inside a mitochondrion. Inputs in boxes on the left: pyruvate (3 carbons). Outputs in boxes on the right: acetyl CoA (2 carbons); CO2 (1 carbon); NADH. No wavy arrow label is drawn.

  6. Bo reads a model where pyruvate loses one carbon as CO2 while NADH forms. Which stage is this, and where does it happen?

    • Pyruvate oxidation, in the mitochondrial matrix
    • Pyruvate oxidation, in the cytoplasm
    • Glycolysis, in the mitochondrial matrix
    • Electron transport chain, on the inner membrane
  7. Use this

    Flow diagram (model, not to scale), drawn inside a mitochondrion. Inputs in boxes on the left: NADH in the matrix; oxygen at the inner membrane. Outputs in boxes on the right: CO2 leaving the matrix; water forming at the inner membrane. No wavy arrow label is drawn.

  8. Jun reads a diagram with CO2 leaving the matrix and water forming at the inner membrane. Which stage uses oxygen directly?

    • The lungs, which use the oxygen as soon as it is breathed in
    • Glycolysis, which starts by splitting glucose in the cytoplasm
    • The citric acid cycle, which turns oxygen into the CO2 it releases
    • The electron transport chain, which forms water from the oxygen
  9. Read about only: the teacher handles the equipment. Kira reads a respirometer card of pea seeds. Which statement does the card support?

    • The germinating seeds created new energy, which is why they used oxygen
    • The seeds are plants, so the gas changes come from photosynthesis, not respiration
    • Germinating seeds respire more, since tube 1 used more oxygen and released more CO2
    • Dry seeds respire faster, since tube 2 used more oxygen
  10. Glycolysis table (real data, from OpenStax Biology 2e)

    measureper glucose
    ATP spent2
    ATP made4
    net ATP2
    NADH made2
    pyruvate made2
  11. Milo reads the glycolysis table. Which statement does the table support?

    • Glycolysis nets 4 ATP, since the 2 ATP spent are not counted
    • Glycolysis nets 6 ATP, since 2 spent plus 4 made is 6
    • Glycolysis makes about 90 percent of the ATP, since it comes first
    • Glycolysis makes 4 ATP and spends 2, so the net is 2
  12. Real-data note: chemiosmosis makes about 90 percent of the ATP. Noah reads that chemiosmosis makes about 90 percent of the ATP from glucose. A model cell makes 20 ATP from one glucose. How are the 20 shared?

    • 10 from chemiosmosis and 10 from the other stages
    • 20 from chemiosmosis and 0 from the other stages
    • 18 from chemiosmosis and 2 from the other stages
    • 2 from chemiosmosis and 18 from glycolysis
  13. Use this

    Flow diagram (model, not to scale), drawn inside a mitochondrion. Inputs in boxes on the left: 2 pyruvate (3 carbons each). Outputs in boxes on the right: CO2, amounts not shown. Stages shown in order: pyruvate oxidation, then the citric acid cycle. The 6 carbons of one glucose all end as CO2.

  14. Pia follows the 6 carbons of one glucose, and all 6 end up in CO2. Which statement about how the CO2 forms is right?

    • 6 CO2 form at the last stage, when the oxygen picks up carbon from glucose
    • 6 CO2 form: 3 in each of the 2 pyruvate steps and 0 in the citric acid cycle
    • 6 CO2 form: 2 in glycolysis in the cytoplasm and 4 in the electron transport chain
    • 6 CO2 form: 2 as pyruvate is oxidized and 4 in the citric acid cycle
  15. ATP per glucose card (real data)

    sourceATP per glucose in aerobic respiration
    Judge and Dodd 2020about 30 to 32
    OpenStax Biology 2ethe number varies
  16. Ren reads that the ATP yield per glucose varies. What can make the number vary from cell to cell?

    • The number of hydrogen ions pumped and how electrons enter the mitochondrion
    • The number of breaths the person takes in a minute
    • How much extra energy the cell creates beyond what glucose holds
    • Glycolysis nets 2 ATP in some cells and 30 ATP in others
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9th grade Cellular respiration: the equation and its three stages worksheet – printable
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About this worksheet

Cellular respiration: the equation and its three stages. Every version has 10 questions, and the answer key prints on the second page.

What your child will learn

  • I can write the overall equation C6H12O6 + 6O2 → 6CO2 + 6H2O + energy, name the inputs and outputs, and count the atoms on both sides (6 C, 12 H and 18 O on each side).
  • I can say that the energy in the equation is not new: it is energy that glucose already held, moved into ATP and heat. I can say a cell does not create energy.
  • I can name the three stages and place each one: glycolysis in the cytoplasm; pyruvate oxidation and the citric acid cycle in the mitochondrial matrix; the electron transport chain and chemiosmosis on the inner mitochondrial membrane.
  • I can say what goes in and comes out of glycolysis (1 glucose in; 2 pyruvate out; 2 ATP spent and 4 made, so a net of 2 ATP; 2 NADH) and where the CO2 is released (2 CO2 as pyruvate is oxidized, 4 in the citric acid cycle, 6 in all).
  • I can follow the oxygen: it is used only in the electron transport chain, where it takes up electrons and hydrogen ions and forms water. I can say the CO2 carbon comes from glucose, not from the oxygen.
  • I can say most of the ATP from glucose (about 90 percent) is made by chemiosmosis on the inner membrane, that glycolysis gives a small net share, and that the total per glucose varies (about 30 to 32 in aerobic respiration).

Standard: HS-LS1-7

Words to know

cellular respiration: Cells using glucose and oxygen to make ATP, giving off CO2 and water

glucose: The six-carbon sugar C6H12O6 that cells break down for energy

glycolysis: The first stage: in the cytoplasm, glucose is split into two pyruvate

pyruvate: A three-carbon molecule made when glucose is split

mitochondrion (plural: mitochondria): The double-membrane part where most steps of cellular respiration take place

matrix: The space inside the inner membrane of a mitochondrion

pyruvate oxidation: Pyruvate loses one carbon as CO2 and becomes an acetyl group, forming NADH

citric acid cycle: A loop in the matrix that releases the rest of the carbon as CO2

Common mistakes

Your child thought cellular respiration is the same as breathing. Breathing moves air in and out; cellular respiration is the reaction inside cells that makes ATP. Ask your child where each one happens.

Your child thought plants do not do cellular respiration. Plant cells make glucose by photosynthesis and then break it down in their mitochondria to make ATP. Ask your child which part of a leaf cell makes ATP.

Your child thought cellular respiration creates energy. Energy cannot be created; a cell moves the energy stored in glucose into ATP and heat. Ask your child where the energy was before the reaction.

Questions parents ask

What is the most common mistake with cellular respiration: the equation and its three stages?

Your child thought cellular respiration is the same as breathing. Breathing moves air in and out; cellular respiration is the reaction inside cells that makes ATP. Ask your child where each one happens.

Are these 9th grade science worksheets free to print?

Yes. All 6 printable versions at each level are free to print, with the answer key, and no account is needed.

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