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9th grade Cellular respiration: the equation and its three stages worksheet

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Cellular respiration: the equation and its three stages

Grade 9 Science Worksheet - Challenge Me

NameDate

Circle the best answer.

  1. Read this first

    Use this

    Flow diagram (model, not to scale), drawn across a cell. Inputs in boxes on the left: 1 glucose (6 carbons). Outputs in boxes on the right: 2 acetyl groups (2 carbons each); CO2 released. Stage 1, in the cytoplasm: glucose becomes 2 pyruvate (3 carbons each). Stage 2, in the mitochondrial matrix: each pyruvate loses 1 carbon as CO2 and becomes an acetyl group.

  2. Pia tracks the carbons of 1 glucose through glycolysis and then pyruvate oxidation. How many CO2 have left, and how many carbons are in the acetyl groups?

    • 2 CO2 have left in the matrix, and 6 carbons are in the acetyl groups
    • 4 CO2 have left in the matrix, and 2 carbons are in the acetyl groups
    • 2 CO2 left in the cytoplasm during glycolysis, and 4 carbons are in the acetyl groups
    • 2 CO2 have left in the matrix, and 4 carbons are in the acetyl groups
  3. Use this

    Flow diagram (model, not to scale), drawn on the inner mitochondrial membrane. Inputs in boxes on the left: oxygen, O2 (atoms labeled); electrons and hydrogen ions from NADH. Outputs in boxes on the right: water. A wavy arrow points out: energy used to make ATP. Earlier stages release CO2 in the matrix, and they do not use oxygen directly.

  4. In a model, Ren puts a label on the oxygen atoms in O2 and follows them. According to the last stage of respiration, where do the labeled atoms end up?

    • In the ATP made by ATP synthase
    • In the water formed at the electron transport chain
    • In the glucose that the cell rebuilds
    • In the CO2 released by the citric acid cycle
  5. Use this

    Flow diagram (model, not to scale), drawn inside a cell. Inputs in boxes on the left: glucose, amount not shown; oxygen, amount not shown. Outputs in boxes on the right: 18 CO2 released (model data); water.

  6. A model cell releases 18 CO2 as it breaks down glucose by cellular respiration. Zee uses the equation. How many glucose were broken down, and how many O2 were used?

    • 3 glucose and 3 O2
    • 18 glucose and 18 O2
    • 6 glucose and 36 O2
    • 3 glucose and 18 O2
  7. Read about only: the teacher handles the equipment. Ari's class adds tube C with 10 g of the same germinating bean seeds, kept in the same conditions. Which prediction fits the card?

    • About 12.0 mL of oxygen and 0 mL of CO2, since plant cells use up carbon dioxide
    • About 6.0 mL of each gas, because the same kind of seeds respire at the same rate
    • About 12.0 mL of oxygen and 24.0 mL of CO2, since respiration creates extra energy and gas
    • About 12.0 mL of oxygen and 12.0 mL of CO2, since twice as many cells respire
  8. In this model cell, chemiosmosis makes exactly 90 percent of the ATP. Esme reads a model cell. Chemiosmosis made 27 ATP from one glucose, exactly 90 percent of all its ATP. How many ATP did the cell make in all?

    • 24, because 27 × 0.9 is about 24
    • 3, because the other 10 percent is 3
    • 30, because 27 is 90 percent of 30
    • 29, because glycolysis adds a net 2 ATP to the 27
  9. Notebook lines (model data)

    linenotebook says
    1Glycolysis happens in the cytoplasm
    2Pyruvate is oxidized to an acetyl group in the mitochondrial matrix
    3The citric acid cycle happens in the cytoplasm
    4The electron transport chain sits in the inner mitochondrial membrane
  10. Jun's notebook lists four lines about cellular respiration. One line has a flaw. Which line has the flaw?

    • Line 3: the citric acid cycle happens in the matrix, not the cytoplasm
    • Line 2: pyruvate is oxidized in the cytoplasm, not the matrix
    • Line 1: glycolysis happens in the mitochondrial matrix, not the cytoplasm
    • Line 4: the chain sits in the matrix, not the inner membrane
  11. Use this

    Notebook line (model data), 1 row. Columns: line, notebook says. 1, Glycolysis nets 4 ATP per glucose, which is most of the ATP a cell makes.

  12. Kira's notebook says glycolysis nets 4 ATP per glucose, which is most of the ATP a cell makes. What is the flaw?

    • Glycolysis nets 6 ATP, and chemiosmosis makes the rest
    • Glycolysis nets 2 ATP, and the lungs make about 90 percent of the ATP
    • Glycolysis nets 4 ATP, and the later stages add a few more
    • Glycolysis nets 2 ATP, and chemiosmosis makes about 90 percent of the ATP
  13. Breathing and muscle card (model data)

    momentbreaths per minuteoxygen used by leg muscle cells (relative units)
    resting131
    after 3 minutes of fast walking223
    after 2 minutes of rest151
  14. Luz says the card proves breathing is cellular respiration, because both numbers rise together. What is the flaw?

    • Nothing is wrong, since breathing is how cells make ATP
    • Breathing moves air in and out; cellular respiration is the reaction in cells
    • The card shows muscle cells created extra energy, not respiration
    • Cellular respiration happens in the lungs, which speed up with breathing
  15. “Flow diagram (model, not to scale)” Milo says leaf cells make their own glucose, so they do not need cellular respiration. Which reply is correct?

    • Agree: plant cells make food, so they skip the breakdown of glucose
    • Disagree: plant mitochondria break sugars down with oxygen to make ATP
    • Disagree: leaves breathe through tiny openings, and that breathing is their cellular respiration
    • Agree: plants use light for their ATP, so mitochondria are not needed
  16. Use this

    Notebook page with a flow diagram (model, not to scale), drawn across a cell. Inputs in boxes on the left: 6 O2, which holds 12 oxygen atoms. Outputs in boxes on the right: 6 CO2, which holds 12 oxygen atoms. Last stage, on the inner mitochondrial membrane: oxygen accepts electrons and forms water.

  17. Tala counts 12 oxygen atoms in 6 O2 and 12 in 6 CO2, and says the oxygen a cell takes in becomes its CO2. Which reply is correct?

    • Disagree: oxygen forms water at the last stage, and the CO2 carbon comes from glucose
    • Agree: the counts match, so the O2 oxygen moves into the CO2
    • Disagree: the CO2 is released by glycolysis in the cytoplasm
    • Disagree: the oxygen is used in the lungs, so cells make CO2 without it
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9th grade Cellular respiration: the equation and its three stages worksheet – printable
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About this worksheet

Cellular respiration: the equation and its three stages. Every version has 10 questions, and the answer key prints on the second page.

What your child will learn

  • I can write the overall equation C6H12O6 + 6O2 → 6CO2 + 6H2O + energy, name the inputs and outputs, and count the atoms on both sides (6 C, 12 H and 18 O on each side).
  • I can say that the energy in the equation is not new: it is energy that glucose already held, moved into ATP and heat. I can say a cell does not create energy.
  • I can name the three stages and place each one: glycolysis in the cytoplasm; pyruvate oxidation and the citric acid cycle in the mitochondrial matrix; the electron transport chain and chemiosmosis on the inner mitochondrial membrane.
  • I can say what goes in and comes out of glycolysis (1 glucose in; 2 pyruvate out; 2 ATP spent and 4 made, so a net of 2 ATP; 2 NADH) and where the CO2 is released (2 CO2 as pyruvate is oxidized, 4 in the citric acid cycle, 6 in all).
  • I can follow the oxygen: it is used only in the electron transport chain, where it takes up electrons and hydrogen ions and forms water. I can say the CO2 carbon comes from glucose, not from the oxygen.
  • I can say most of the ATP from glucose (about 90 percent) is made by chemiosmosis on the inner membrane, that glycolysis gives a small net share, and that the total per glucose varies (about 30 to 32 in aerobic respiration).

Standard: HS-LS1-7

Words to know

cellular respiration: Cells using glucose and oxygen to make ATP, giving off CO2 and water

glucose: The six-carbon sugar C6H12O6 that cells break down for energy

glycolysis: The first stage: in the cytoplasm, glucose is split into two pyruvate

pyruvate: A three-carbon molecule made when glucose is split

mitochondrion (plural: mitochondria): The double-membrane part where most steps of cellular respiration take place

matrix: The space inside the inner membrane of a mitochondrion

pyruvate oxidation: Pyruvate loses one carbon as CO2 and becomes an acetyl group, forming NADH

citric acid cycle: A loop in the matrix that releases the rest of the carbon as CO2

Common mistakes

Your child thought cellular respiration is the same as breathing. Breathing moves air in and out; cellular respiration is the reaction inside cells that makes ATP. Ask your child where each one happens.

Your child thought plants do not do cellular respiration. Plant cells make glucose by photosynthesis and then break it down in their mitochondria to make ATP. Ask your child which part of a leaf cell makes ATP.

Your child thought cellular respiration creates energy. Energy cannot be created; a cell moves the energy stored in glucose into ATP and heat. Ask your child where the energy was before the reaction.

Questions parents ask

What is the most common mistake with cellular respiration: the equation and its three stages?

Your child thought cellular respiration is the same as breathing. Breathing moves air in and out; cellular respiration is the reaction inside cells that makes ATP. Ask your child where each one happens.

Are these 9th grade science worksheets free to print?

Yes. All 6 printable versions at each level are free to print, with the answer key, and no account is needed.

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