Cellular respiration: the equation and its three stages
Grade 9 Science Worksheet - Building Confidence

Circle the best answer.
“model, not to scale” Ari reads a diagram of one stage of breaking down glucose. In box A, 1 glucose is split into 2 pyruvate. Where in the cell does stage A happen?
- In the cytoplasm
- In the mitochondrial matrix
- On the inner mitochondrial membrane
- In the nucleus
Flow diagram (model, not to scale), drawn across a cell with one mitochondrion. No wavy arrow label is drawn. Bo reads a diagram of the citric acid cycle. In box B, an acetyl group joins a four-carbon molecule and CO2 is released. Where does stage B happen?
Flow diagram (model, not to scale), drawn across a cell with one mitochondrion. Inputs in boxes on the left: acetyl group (2 carbons); four-carbon molecule. Outputs in boxes on the right: B: CO2. No wavy arrow label is drawn. The places shown are the cytoplasm, the matrix and inner membrane of the mitochondrion, and a ribosome next to the nucleus. Flow diagram (model, not to scale), drawn across a cell with one mitochondrion. Inputs in boxes on the left: acetyl group (2 carbons); four-carbon molecule. Outputs in boxes on the right: B: CO2. No wavy arrow label is drawn. The places shown are the cytoplasm, the matrix and inner membrane of the mitochondrion, and a ribosome next to the nucleus. nucleus ribosome cytoplasm matrix inner membrane acetyl group (2 carbons) four-carbon molecule B: CO2 - In the mitochondrial matrix
- In the cytoplasm outside the mitochondrion
- On the inner mitochondrial membrane
- On a ribosome next to the nucleus
Read this first
Use this
Flow diagram (model, not to scale), drawn across a cell with one mitochondrion. Inputs in boxes on the left: electrons from NADH; oxygen. Outputs in boxes on the right: water. Box C is the chain of proteins. A wavy arrow points out: energy used to make ATP.
Esme reads that oxygen is used at box C, a chain of proteins that passes electrons along. Where is this electron transport chain found?
- In the cytoplasm outside the mitochondrion
- In the matrix inside the mitochondrion
- On the inner mitochondrial membrane
- In a vacuole near the cell membrane
Equation card, from OpenStax Biology 2e
C6H12O6 + 6O2 → 6CO2 + 6H2O + energy. Inputs in boxes on the left: glucose, C6H12O6; oxygen, 6O2. Outputs in boxes on the right: carbon dioxide, 6CO2; water, 6H2O. A wavy arrow points out: energy. The cell is a leaf cell kept in the dark.
Jun reads that a leaf cell kept in the dark breaks down glucose to make ATP. Which two things does the respiration equation show going in?
- Light and carbon dioxide
- Glucose and carbon dioxide
- Carbon dioxide and water
- Glucose and oxygen
Equation card, from OpenStax Biology 2e
C6H12O6 + 6O2 → 6CO2 + 6H2O + energy. Inputs in boxes on the left: glucose, C6H12O6; oxygen, 6O2. Outputs in boxes on the right: carbon dioxide, 6CO2; water, 6H2O. A wavy arrow points out: energy.
Kira reads the respiration equation. How many oxygen molecules, O2, does the equation use for each glucose?
- 12, the count of oxygen atoms instead
- 2, the small number after the O
- 6, the large number in front of the O2
- 1, the same count as the glucose
Flow diagram (model, not to scale), drawn on the inner membrane of a mitochondrion. Luz follows oxygen to the last stage. Oxygen picks up electrons and hydrogen ions at the end of the chain. What product forms in box D?
Flow diagram (model, not to scale), drawn on the inner membrane of a mitochondrion. Inputs in boxes on the left: oxygen, O2; electrons and hydrogen ions. Outputs in boxes on the right: D. A wavy arrow points out: energy used to make ATP. Flow diagram (model, not to scale), drawn on the inner membrane of a mitochondrion. Inputs in boxes on the left: oxygen, O2; electrons and hydrogen ions. Outputs in boxes on the right: D. A wavy arrow points out: energy used to make ATP. inner membrane of a mitochondrion oxygen, O2 electrons and hydrogen ions D energy used to make ATP - Water, H2O
- Glucose, C6H12O6
- Pyruvate, from glycolysis
- Carbon dioxide, CO2
Flow diagram (model, not to scale), drawn across a cell. Milo hears that cells carry out cellular respiration. Which phrase tells what cellular respiration means?
Flow diagram (model, not to scale), drawn across a cell. Inputs in boxes on the left: glucose; oxygen. Outputs in boxes on the right: carbon dioxide; water. A wavy arrow points out: energy as ATP and heat. Flow diagram (model, not to scale), drawn across a cell. Inputs in boxes on the left: glucose; oxygen. Outputs in boxes on the right: carbon dioxide; water. A wavy arrow points out: energy as ATP and heat. cell glucose oxygen carbon dioxide water energy as ATP and heat - Making glucose from light, water and carbon dioxide
- Using glucose and oxygen in cells to make ATP
- Breathing air in and out through the lungs
- Making new energy inside the cell from nothing
Glycolysis ATP count (real data, from OpenStax Biology 2e)
measure ATP per glucose ATP spent 2 ATP made 4 Noah reads that glycolysis spends 2 ATP and makes 4 ATP from one glucose. Which phrase describes the ATP glycolysis adds for the cell?
- A small net gain of 2 ATP per glucose
- A net gain of 4 ATP, with nothing spent
- Most of the ATP made from one glucose
- ATP made by the electron transport chain
Ren reads a chart of where the ATP from glucose is made. Blank box D makes about 90 percent of the ATP. Which label fits D?
Chart of the ATP made from one glucose (real data, from OpenStax Biology 2e), 2 rows. Columns: box, share of the ATP. D, about 90 percent; E, a small share. The label of box D is blank. Chart of the ATP made from one glucose (real data, from OpenStax Biology 2e), 2 rows. Columns: box, share of the ATP. D, about 90 percent; E, a small share. The label of box D is blank. ATP made from one glucose (real data, from OpenStax Biology 2e) box share of the ATP D about 90 percent E a small share The label of box D is blank. - Chemiosmosis on the inner membrane
- The citric acid cycle in the matrix
- Pyruvate oxidation in the matrix
- Glycolysis in the cytoplasm
No wavy arrow label is drawn. Zee sees pyruvate broken down in the mitochondrial matrix, with a gas leaving at box G. The gas is made from pyruvate's carbon. Which label fits G?
Flow diagram (model, not to scale), drawn inside a mitochondrion. Inputs in boxes on the left: pyruvate. Outputs in boxes on the right: acetyl group; G: a gas that leaves the matrix. No wavy arrow label is drawn. Flow diagram (model, not to scale), drawn inside a mitochondrion. Inputs in boxes on the left: pyruvate. Outputs in boxes on the right: acetyl group; G: a gas that leaves the matrix. No wavy arrow label is drawn. Flow diagram (model, not to scale) matrix mitochondrion pyruvate acetyl group G - Oxygen, the same gas the cell took in
- Glucose, rebuilt from the pyruvate
- Carbon dioxide, with carbon from pyruvate
- Water vapor, formed from the pyruvate

