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9th grade Cellular respiration: the equation and its three stages worksheet

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Cellular respiration: the equation and its three stages

Grade 9 Science Worksheet - Building Confidence

NameDate

Circle the best answer.

  1. “model, not to scale” Ari reads a diagram of one stage of breaking down glucose. In box A, 1 glucose is split into 2 pyruvate. Where in the cell does stage A happen?

    • In the cytoplasm
    • In the mitochondrial matrix
    • On the inner mitochondrial membrane
    • In the nucleus
  2. Flow diagram (model, not to scale), drawn across a cell with one mitochondrion. No wavy arrow label is drawn. Bo reads a diagram of the citric acid cycle. In box B, an acetyl group joins a four-carbon molecule and CO2 is released. Where does stage B happen?

    • In the mitochondrial matrix
    • In the cytoplasm outside the mitochondrion
    • On the inner mitochondrial membrane
    • On a ribosome next to the nucleus
  3. Read this first

    Use this

    Flow diagram (model, not to scale), drawn across a cell with one mitochondrion. Inputs in boxes on the left: electrons from NADH; oxygen. Outputs in boxes on the right: water. Box C is the chain of proteins. A wavy arrow points out: energy used to make ATP.

  4. Esme reads that oxygen is used at box C, a chain of proteins that passes electrons along. Where is this electron transport chain found?

    • In the cytoplasm outside the mitochondrion
    • In the matrix inside the mitochondrion
    • On the inner mitochondrial membrane
    • In a vacuole near the cell membrane
  5. Equation card, from OpenStax Biology 2e

    C6H12O6 + 6O2 → 6CO2 + 6H2O + energy. Inputs in boxes on the left: glucose, C6H12O6; oxygen, 6O2. Outputs in boxes on the right: carbon dioxide, 6CO2; water, 6H2O. A wavy arrow points out: energy. The cell is a leaf cell kept in the dark.

  6. Jun reads that a leaf cell kept in the dark breaks down glucose to make ATP. Which two things does the respiration equation show going in?

    • Light and carbon dioxide
    • Glucose and carbon dioxide
    • Carbon dioxide and water
    • Glucose and oxygen
  7. Equation card, from OpenStax Biology 2e

    C6H12O6 + 6O2 → 6CO2 + 6H2O + energy. Inputs in boxes on the left: glucose, C6H12O6; oxygen, 6O2. Outputs in boxes on the right: carbon dioxide, 6CO2; water, 6H2O. A wavy arrow points out: energy.

  8. Kira reads the respiration equation. How many oxygen molecules, O2, does the equation use for each glucose?

    • 12, the count of oxygen atoms instead
    • 2, the small number after the O
    • 6, the large number in front of the O2
    • 1, the same count as the glucose
  9. Flow diagram (model, not to scale), drawn on the inner membrane of a mitochondrion. Luz follows oxygen to the last stage. Oxygen picks up electrons and hydrogen ions at the end of the chain. What product forms in box D?

    • Water, H2O
    • Glucose, C6H12O6
    • Pyruvate, from glycolysis
    • Carbon dioxide, CO2
  10. Flow diagram (model, not to scale), drawn across a cell. Milo hears that cells carry out cellular respiration. Which phrase tells what cellular respiration means?

    • Making glucose from light, water and carbon dioxide
    • Using glucose and oxygen in cells to make ATP
    • Breathing air in and out through the lungs
    • Making new energy inside the cell from nothing
  11. Glycolysis ATP count (real data, from OpenStax Biology 2e)

    measureATP per glucose
    ATP spent2
    ATP made4
  12. Noah reads that glycolysis spends 2 ATP and makes 4 ATP from one glucose. Which phrase describes the ATP glycolysis adds for the cell?

    • A small net gain of 2 ATP per glucose
    • A net gain of 4 ATP, with nothing spent
    • Most of the ATP made from one glucose
    • ATP made by the electron transport chain
  13. Ren reads a chart of where the ATP from glucose is made. Blank box D makes about 90 percent of the ATP. Which label fits D?

    • Chemiosmosis on the inner membrane
    • The citric acid cycle in the matrix
    • Pyruvate oxidation in the matrix
    • Glycolysis in the cytoplasm
  14. No wavy arrow label is drawn. Zee sees pyruvate broken down in the mitochondrial matrix, with a gas leaving at box G. The gas is made from pyruvate's carbon. Which label fits G?

    • Oxygen, the same gas the cell took in
    • Glucose, rebuilt from the pyruvate
    • Carbon dioxide, with carbon from pyruvate
    • Water vapor, formed from the pyruvate
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9th grade Cellular respiration: the equation and its three stages worksheet – printable
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About this worksheet

Cellular respiration: the equation and its three stages. Every version has 10 questions, and the answer key prints on the second page.

What your child will learn

  • I can write the overall equation C6H12O6 + 6O2 → 6CO2 + 6H2O + energy, name the inputs and outputs, and count the atoms on both sides (6 C, 12 H and 18 O on each side).
  • I can say that the energy in the equation is not new: it is energy that glucose already held, moved into ATP and heat. I can say a cell does not create energy.
  • I can name the three stages and place each one: glycolysis in the cytoplasm; pyruvate oxidation and the citric acid cycle in the mitochondrial matrix; the electron transport chain and chemiosmosis on the inner mitochondrial membrane.
  • I can say what goes in and comes out of glycolysis (1 glucose in; 2 pyruvate out; 2 ATP spent and 4 made, so a net of 2 ATP; 2 NADH) and where the CO2 is released (2 CO2 as pyruvate is oxidized, 4 in the citric acid cycle, 6 in all).
  • I can follow the oxygen: it is used only in the electron transport chain, where it takes up electrons and hydrogen ions and forms water. I can say the CO2 carbon comes from glucose, not from the oxygen.
  • I can say most of the ATP from glucose (about 90 percent) is made by chemiosmosis on the inner membrane, that glycolysis gives a small net share, and that the total per glucose varies (about 30 to 32 in aerobic respiration).

Standard: HS-LS1-7

Words to know

cellular respiration: Cells using glucose and oxygen to make ATP, giving off CO2 and water

glucose: The six-carbon sugar C6H12O6 that cells break down for energy

glycolysis: The first stage: in the cytoplasm, glucose is split into two pyruvate

pyruvate: A three-carbon molecule made when glucose is split

mitochondrion (plural: mitochondria): The double-membrane part where most steps of cellular respiration take place

matrix: The space inside the inner membrane of a mitochondrion

pyruvate oxidation: Pyruvate loses one carbon as CO2 and becomes an acetyl group, forming NADH

citric acid cycle: A loop in the matrix that releases the rest of the carbon as CO2

Common mistakes

Your child thought cellular respiration is the same as breathing. Breathing moves air in and out; cellular respiration is the reaction inside cells that makes ATP. Ask your child where each one happens.

Your child thought plants do not do cellular respiration. Plant cells make glucose by photosynthesis and then break it down in their mitochondria to make ATP. Ask your child which part of a leaf cell makes ATP.

Your child thought cellular respiration creates energy. Energy cannot be created; a cell moves the energy stored in glucose into ATP and heat. Ask your child where the energy was before the reaction.

Questions parents ask

What is the most common mistake with cellular respiration: the equation and its three stages?

Your child thought cellular respiration is the same as breathing. Breathing moves air in and out; cellular respiration is the reaction inside cells that makes ATP. Ask your child where each one happens.

Are these 9th grade science worksheets free to print?

Yes. All 6 printable versions at each level are free to print, with the answer key, and no account is needed.

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