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9th grade Diffusion and osmosis worksheet

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Diffusion and osmosis

Grade 9 Science Worksheet - Building Confidence

NameDate

Circle the best answer.

  1. Membrane drawing (model, not to scale). No proteins are drawn. The two sides are the same size. Ari reads the term concentration gradient beside a drawing. What does a concentration gradient mean?

    • The point where a substance stops moving in a space
    • The energy a cell spends to move a substance across a membrane
    • A difference in how crowded a substance is between two places
    • A tunnel in a protein that lets a substance cross a membrane
  2. Read this first

    Use this

    Membrane drawing (model, not to scale). No proteins are drawn.

  3. A label on Bo's drawing says passive transport. What does passive transport mean?

    • Substances cross through proteins that use energy from the cell
    • Substances stay on one side because the membrane blocks them
    • Substances move across a membrane and the cell spends no energy
    • Substances move across a membrane using energy the cell supplies
  4. Both sides of Esme's drawing hold 5 dots, and a label says dynamic equilibrium. What does that mean?

    • The dots gather on one side until the membrane is full
    • Each dot keeps moving in one direction, from outside to inside
    • The dots have stopped moving because the two sides match
    • The dots keep moving, but there is no net movement either way
  5. Membrane drawing (model, not to scale). No proteins are drawn. The two sides are the same size. In Jun's drawing, the two sides are the same size. Which side has the higher concentration of oxygen?

    • Inside the cell, which holds 12 molecules to 3 outside it
    • Outside the cell, which holds 12 molecules to 3 inside
    • Neither side, because the two sides are the same size
    • Inside the cell, because it is behind the membrane
  6. Use this

    Membrane drawing (model, not to scale). A bilayer of phospholipids with watery fluid outside the cell above and watery fluid inside the cell below. Letter C points to a protein that reaches all the way across the bilayer and changes shape to carry glucose. Outside the cell: 11 glucose molecules. Inside the cell: 4 glucose molecules.

  7. Kira reads a drawing of glucose near carrier protein C. How many more glucose molecules are outside the cell than inside?

    • 4 more glucose molecules outside
    • 7 more glucose molecules outside
    • 11 more glucose molecules outside
    • 15 more glucose molecules outside
  8. Two membrane drawings (model, not to scale). Noah compares two drawings of oxygen crossing a membrane. Which drawing best shows how the molecules move?

    • Drawing 2, because the cell spends energy to send molecules inward
    • Drawing 1, because molecules move both ways, with more going inward
    • Neither drawing, because molecules cannot cross without a protein
    • Drawing 2, because each molecule moves from the crowded side to the other
  9. Use this

    Two lab membrane drawings (model, not to scale). Each has a selectively permeable membrane with fluid on side 1 above and fluid on side 2 below. Water crosses the membrane; solute dots cannot. Side 1 holds 2 solute dots and side 2 holds 8 solute dots, in both drawings. Drawing 1: the water arrow points from side 1 to side 2. Drawing 2: the water arrow points from side 2 to side 1.

  10. Pia compares two drawings of a lab membrane that passes water but not solute. Which drawing shows the way water moves?

    • Drawing 1, because the solute dots follow the water arrow across
    • Drawing 1, because water moves toward the side with more solute
    • Neither drawing, because the solute dots block the water
    • Drawing 2, because water moves toward the side with less solute
  11. Use this

    Membrane drawing (model, not to scale). A bilayer of phospholipids with watery fluid outside the cell above and watery fluid inside the cell below. No proteins are drawn. Outside the cell: 4 carbon dioxide molecules. Inside the cell: 13 carbon dioxide molecules. The two sides are the same size.

  12. Ren watches a model of carbon dioxide near a cell membrane. Which way do the molecules move on net, and does the cell spend energy?

    • Into the cell, from fewer molecules to more, with no energy from the cell
    • Out of the cell, down the gradient, with no energy from the cell
    • Out of the cell, with no molecule moving in at the same time
    • Out of the cell, down the gradient, powered by energy from the cell
  13. Use this

    Lab membrane drawing (model, not to scale). A selectively permeable membrane with fluid on side 1 above and fluid on side 2 below. Water crosses the membrane; solute dots cannot. Side 1 holds 3 solute dots and side 2 holds 9 solute dots. The two sides are the same size.

  14. Tala's class reads a lab membrane that passes water but not solute. Which way does water move across it on net?

    • From side 2 to side 1, the side with less solute
    • Water stays put, because the membrane stops the solute dots
    • From side 1 to side 2, the side with more solute
    • Both ways equally, because water crosses in both directions
  15. Membrane drawing (model, not to scale). Zee reads a drawing of sodium ions and channel protein J. Which statement about sodium ions crossing through J is right?

    • They move in through J, down the gradient, with no cell energy
    • They slip between the tails, so J is not needed
    • They move out through J, from fewer ions to more
    • They move in through J, but the cell must spend energy to use it
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9th grade Diffusion and osmosis worksheet – printable
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About this worksheet

Diffusion and osmosis. Every version has 10 questions, and the answer key prints on the second page.

What your child will learn

  • I can say what a concentration gradient is, and read which side of a drawing or table is more concentrated.
  • I can say that diffusion is the net movement of a substance from higher to lower concentration, that it is passive (the cell spends no energy), and that it goes on until there is no net movement.
  • I can say what dynamic equilibrium is: particles keep moving both ways, but there is no net change.
  • I can name the factors that change the rate (the size of the gradient, temperature, the mass of the particles, the density of the solvent, the distance) and predict which way each one pushes the rate.
  • I can tell simple diffusion (between the tails) from facilitated diffusion (through a channel or carrier protein), and say that both are passive and both go down the gradient.
  • I can say that osmosis is the diffusion of water across a selectively permeable membrane, toward the side with more solute (less free water), and predict which side of a U-tube gains water.

Standard: supports HS-LS1-3

Words to know

concentration: How much of a substance there is in a given space

concentration gradient: A difference in concentration between two places

diffusion: Net movement of a substance from higher to lower concentration

passive: Needs no energy from the cell

dynamic equilibrium: Particles keep moving, but there is no net movement

net movement: The overall change left over after movement in both directions

facilitated diffusion: Diffusion through a channel or carrier protein, still passive

channel protein: A protein with a tunnel for one kind of particle

Common mistakes

Your child thought particles stop moving once the amounts are equal. At equilibrium they keep moving both ways, but there is no net change. Ask what the dots are doing when the counts stay the same.

Your child thought the cell spends energy to make diffusion happen. Diffusion is passive: the particles' own motion down the gradient does the work. Ask who supplies the energy when a gas spreads out.

Your child had water moving toward the side with less dissolved solute. In osmosis water moves toward the side with more solute, where there is less free water. Ask which side has more free water and where the water goes.

Questions parents ask

What is the most common mistake with diffusion and osmosis?

Your child thought particles stop moving once the amounts are equal. At equilibrium they keep moving both ways, but there is no net change. Ask what the dots are doing when the counts stay the same.

Are these 9th grade science worksheets free to print?

Yes. All 6 printable versions at each level are free to print, with the answer key, and no account is needed.

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