Diffusion and osmosis
Grade 9 Science Worksheet - Challenge Me

Circle the best answer.
Ari reads a graph of particles on two sides of a membrane they cross. What does the flat part of both lines, after 30 minutes, show?
- The membrane ran out of energy for moving particles at 30 minutes
- Particles crossed from side 1 to side 2, and no particle crossed back
- Particles stopped crossing once the sides reached 50
- The sides are equal near 50, with particles still crossing both ways
Esme reads a graph of one substance moving into a lab chamber. The line climbs steeply, then flattens. Why does the climb slow down?
Graph (model data). Concentration in a lab chamber that a substance crosses into from a second chamber of the same size, which started at 80. Time (minutes) across, 0 to 30, a mark every 5. Concentration (units) up, 0 to 40, a mark every 10. The line joins (0, 0), (5, 20), (10, 30), (15, 35), (20, 38), (25, 39), (30, 40) with straight pieces. Graph (model data). Concentration in a lab chamber that a substance crosses into from a second chamber of the same size, which started at 80. Time (minutes) across, 0 to 30, a mark every 5. Concentration (units) up, 0 to 40, a mark every 10. The line joins (0, 0), (5, 20), (10, 30), (15, 35), (20, 38), (25, 39), (30, 40) with straight pieces. Graph (model data). Concentration in a lab chamber that a substance crosses into from a second chamber of the same size, which started at 80. 0 5 10 15 20 25 30 0 10 20 30 40 Time (minutes) Concentration (units) - Particles slow down and stop moving as the line flattens
- The chamber is running low on energy to power diffusion
- Particles cross into the chamber and do not cross back, so the first empties
- The gradient shrinks as the chambers near equal, so diffusion slows
Read this first
Use this
Lab card (model data). A bag of dialysis tubing, a kind of lab membrane, held starch molecules and sat in a beaker with iodine molecules. The lab membrane lets iodine molecules cross but not starch molecules. Table with 2 rows. Columns: Particle | In the bag at 0 minutes | In the bag at 30 minutes | In the beaker at 30 minutes. Rows: starch | 10 | 10 | 0; iodine | 0 | 7 | 3. The beaker held 10 iodine molecules and no starch at 0 minutes. A teacher ran the test; read about only.
Jun reads a teacher's card about a bag of dialysis tubing, a lab membrane, set in a beaker. Which conclusion does the card support?
- Iodine moved into the bag, and no iodine molecule moved back out again
- Starch moved out of the bag down its gradient as iodine moved in
- Iodine moved into the bag down its gradient; starch stayed inside
- Iodine moved into the bag because the membrane spent energy moving it
Use this
Lab card (model data). A U-tube had a membrane across the bottom that passes water but not sugar. The left arm held 3 sugar dots and the right arm held 9 at the start, and both levels began at 20 mL. Table with 3 rows. Columns: Time (minutes) | Left level (mL) | Right level (mL). Rows: 0 | 20 | 20; 10 | 17 | 23; 20 | 15 | 25. A teacher ran the test; read about only.
Kira reads a card for a U-tube with a membrane that passes water but not sugar. Which conclusion does the card support?
- Water moved right to left, toward less sugar, as the right level rose
- Water moved left to right, toward more sugar, as the left level fell 3 mL by 20 minutes
- Sugar dots crossed to the right arm and raised its level
- Water moved left to right, toward more sugar, as the left level fell 5 mL by 20 minutes
Use this
Lab card (model data). A lab-made bilayer held 10 carrier proteins for glucose. The card gives the glucose outside and the rate of crossing. Table with 4 rows. Columns: Glucose outside (units) | Rate (molecules per second). Rows: 2 | 5; 4 | 9; 8 | 12; 16 | 12. A teacher ran the test; read about only.
Luz reads a card about glucose crossing through carrier proteins. The rate stays at 12 when glucose outside rises from 8 to 16. Why?
- Glucose crossed the bilayer alone at 16 units, so carriers were not needed
- The carriers ran out of cell energy once glucose reached 8 units
- Glucose stopped moving at 8 units, because the sides reached equilibrium
- Every carrier was busy, so more glucose could not raise the rate
Use this
Notes card written by a student. Line 1: Diffusion moves a substance from higher to lower concentration. Line 2: The cell spends ATP to run diffusion. Line 3: Warmer conditions speed diffusion up.
Milo's notes on diffusion have three lines. One line is wrong. Which correction fixes the notes?
- Line 3 is wrong: warmer conditions slow diffusion down
- Line 2 is right: diffusion through proteins also spends ATP
- Line 1 is wrong: substances move from lower to higher concentration
- Line 2 is wrong: diffusion is passive, so the cell spends no ATP
Pia's notes say a molecule in diffusion only moves from high to low concentration, so it never moves back. Which correction fixes the notes?
Notes card and membrane drawing (model, not to scale). A bilayer of phospholipids with watery fluid outside the cell above and watery fluid inside the cell below. No proteins are drawn. Outside the cell: 12 oxygen molecules. Inside the cell: 4 oxygen molecules. A long arrow points inward and a short arrow points outward. Notes card and membrane drawing (model, not to scale). A bilayer of phospholipids with watery fluid outside the cell above and watery fluid inside the cell below. No proteins are drawn. Outside the cell: 12 oxygen molecules. Inside the cell: 4 oxygen molecules. A long arrow points inward and a short arrow points outward. Notes watery fluid outside the cell watery fluid inside the cell model, not to scale No proteins are drawn. 12 oxygen molecules 4 oxygen molecules O O O O O O O O O O O O O O O O inward outward - Molecules move at random both ways; the net movement is from high to low
- No correction: molecules move from high to low, and a move back needs cell energy
- Molecules move both ways until equilibrium, and then they stop moving
- Molecules move both ways, but more move outward, so the net movement is outward
Ren compares claims about glucose and carrier protein C when both sides hold 8 glucose molecules. Which claim, with its reason, fits?
Membrane drawing (model, not to scale). A bilayer of phospholipids with watery fluid outside the cell above and watery fluid inside the cell below. Letter C points to a protein that reaches all the way across the bilayer and changes shape to carry glucose. Outside the cell: 8 glucose molecules. Inside the cell: 8 glucose molecules. Membrane drawing (model, not to scale). A bilayer of phospholipids with watery fluid outside the cell above and watery fluid inside the cell below. Letter C points to a protein that reaches all the way across the bilayer and changes shape to carry glucose. Outside the cell: 8 glucose molecules. Inside the cell: 8 glucose molecules. model, not to scale watery fluid outside the cell watery fluid inside the cell glucose phospholipids C - Net movement inward, because the carrier points toward the inside
- Net movement inward, because the carrier uses cell energy to pull glucose in
- No net movement of glucose, because the two sides match and the gradient is gone
- No glucose crosses, because the carrier stops working when the sides match
Use the table
Model data (not to scale)
Cell Width (units) Cell 1 10 Cell 2 40 Both cells start with the same oxygen concentration at the surface and none at the center. Oxygen enters by diffusion at the same temperature.
Tala compares two round model cells at the same temperature. Which claim, with its reason, fits for oxygen reaching the center?
- Cell 2 gets oxygen to its center sooner, because a larger cell has a steeper gradient
- Cell 2 gets oxygen to its center sooner, because a larger cell spends more energy
- Cell 1 gets oxygen to its center sooner, because the distance is shorter
- Both cells get oxygen to the center together, because diffusion is passive
Lab drawing (model, not to scale). Zee reads about a bag of lab membrane that passes water but not starch. Which claim about water, with its reason, fits?
Lab drawing (model, not to scale). A bag of lab membrane holds a starch solution with 8 starch dots. The bag sits in a beaker of plain water with no starch. The membrane passes water but not starch. The bag and the beaker hold the same volume of fluid. Lab drawing (model, not to scale). A bag of lab membrane holds a starch solution with 8 starch dots. The bag sits in a beaker of plain water with no starch. The membrane passes water but not starch. The bag and the beaker hold the same volume of fluid. beaker starch solution bag of lab membrane 8 starch dots no starch plain water passes water but not starch same volume of fluid - Water moves into the bag, because the beaker has more free water
- Water moves into the bag, because the membrane spends energy to pull it in
- Water moves out of the bag, because it moves toward the side with less starch
- Water moves into the bag, because water molecules move in one direction