Diffusion and osmosis
Grade 9 Science Worksheet

Circle the best answer.
Read this first
Use this
Lab card (model data). Coloring particles in two chambers joined by a membrane they can cross. Table with 3 rows. Columns: Time (minutes) | Left chamber | Right chamber. Rows: 0 | 24 | 0; 5 | 16 | 8; 10 | 13 | 11. A teacher ran the test; read about only.
Ari's class reads counts of food-coloring particles in two chambers joined by a membrane they can cross. What will the counts be after a long time?
- About 24 in the right chamber, because particles end up on the far side
- About 12 in each chamber, with particles no longer crossing
- About 12 in each chamber, though no particle crosses back to the left
- About 12 in each chamber, with particles still crossing both ways
No proteins are drawn. Esme reads a drawing with two gases near a cell membrane. Each gas crosses the bilayer alone. Which statement predicts the net movement?
- Oxygen moves in, and the cell spends energy to move carbon dioxide out
- Oxygen moves in and carbon dioxide out, each down its own gradient
- Both gases move in, because the outside holds more molecules in total
- Oxygen moves out and carbon dioxide moves in, because gases swap places
Use this
Lab card (model data). Time for food coloring to spread to the far side of a cup. Table with 2 rows. Columns: Water | Time to spread (seconds). Rows: warm water | 30; cold water | 90. The cups were the same size and held the same amount of water. A teacher ran the test; read about only.
Jun's class drops food coloring into two cups of water and times how long the color takes to reach the far side. What does the card show?
- The cups match, because diffusion is passive and needs no energy
- Warmer water slows diffusion, because particles crowd together
- Warmer water speeds diffusion, because particles have more thermal energy
- Cold water spreads color faster, because dense water pushes particles along
Kira compares two cells where oxygen crosses the bilayer alone. Which cell has the faster net diffusion, and why?
Two membrane drawings (model, not to scale), each a bilayer of phospholipids with no proteins. Cell 1: 16 oxygen molecules outside and 2 inside. Cell 2: 8 oxygen molecules outside and 4 inside. The cells and the temperature are the same. Two membrane drawings (model, not to scale), each a bilayer of phospholipids with no proteins. Cell 1: 16 oxygen molecules outside and 2 inside. Cell 2: 8 oxygen molecules outside and 4 inside. The cells and the temperature are the same. Cell 1 Cell 2 outside: 16 oxygen molecules outside: 8 oxygen molecules inside: 2 oxygen molecules inside: 4 oxygen molecules The cells and the temperature are the same. - Cell 1, because the cell spends more energy when the gradient is steep
- Cell 2, because molecules move faster when the sides are closer to equal
- Cell 1, because a larger difference in concentration speeds diffusion
- The rates match, because both cells have more oxygen outside
Use this
Lab card (model data). Time for each substance to spread across a tank. Table with 2 rows. Columns: Substance | Molecule mass | Time to spread (seconds). Rows: L | lighter | 40; H | heavier | 100. Both tanks held the same warm water and the same starting gradient. A teacher ran the test; read about only.
Luz's class reads a card with two substances in the same warm tank. A third substance, M, has molecules heavier than L's but lighter than H's. Predict M's time.
- Between 40 and 100 seconds, since heavier molecules diffuse more slowly
- Exactly 140 seconds, since the two times add together
- Under 40 seconds, since heavier molecules diffuse faster
- Exactly 40 seconds, since the same gradient gives the same time
model, not to scale. Chloride ions cross a cell membrane through channel protein H, from 14 molecules outside to 5 inside. Milo classifies the transport. Which label fits?
Membrane drawing (model, not to scale). A bilayer of phospholipids with watery fluid outside the cell above and watery fluid inside the cell below. Letter H points to a protein that reaches all the way across the bilayer, with a tunnel shaped to fit chloride ions. Outside the cell: 14 chloride ions. Inside the cell: 5 chloride ions. Membrane drawing (model, not to scale). A bilayer of phospholipids with watery fluid outside the cell above and watery fluid inside the cell below. Letter H points to a protein that reaches all the way across the bilayer, with a tunnel shaped to fit chloride ions. Outside the cell: 14 chloride ions. Inside the cell: 5 chloride ions. Membrane drawing watery fluid outside the cell 14 chloride ions phospholipids H watery fluid inside the cell 5 chloride ions - Facilitated diffusion: passive, through a protein, down the gradient
- Osmosis: chloride ions crossing toward the side with more water
- Facilitated diffusion: through a protein, using energy from the cell
- Simple diffusion: passing between the tails, down the concentration gradient
Noah sorts four transport events shown on a membrane drawing. Which event is simple diffusion?
Membrane drawing (model, not to scale). A bilayer of phospholipids with watery fluid outside the cell above and watery fluid inside the cell below. Letter L points to a channel protein and letter M points to a carrier protein, both reaching all the way across the bilayer. Oxygen: 12 outside and 3 inside. Chloride ions: 10 outside and 4 inside. Glucose: 9 outside and 2 inside. Carbon dioxide: 3 outside and 11 inside. Membrane drawing (model, not to scale). A bilayer of phospholipids with watery fluid outside the cell above and watery fluid inside the cell below. Letter L points to a channel protein and letter M points to a carrier protein, both reaching all the way across the bilayer. Oxygen: 12 outside and 3 inside. Chloride ions: 10 outside and 4 inside. Glucose: 9 outside and 2 inside. Carbon dioxide: 3 outside and 11 inside. Oxygen: 12 Chloride ions: 10 Glucose: 9 Carbon dioxide: 3 watery fluid outside the cell Oxygen: 3 Chloride ions: 4 Glucose: 2 Carbon dioxide: 11 watery fluid inside the cell Membrane drawing (model, not to scale) L M - Carbon dioxide slips between the tails, powered by cell energy
- Chloride ions pass through channel L, down their gradient
- Glucose binds carrier M, which spends cell energy to change shape
- Oxygen slips between the tails, down its gradient, with no protein
Lab membrane drawing (model, not to scale). Pia reads a drawing of aquaporin channels in a lab membrane. Water crosses, but solute cannot. Which label fits the water movement?
Lab membrane drawing (model, not to scale). A selectively permeable membrane with fluid on side 1 above and fluid on side 2 below. Letter A points to an aquaporin, a channel protein that lets water cross. Solute dots cannot cross. Side 1 holds 4 solute dots and side 2 holds 10 solute dots. Lab membrane drawing (model, not to scale). A selectively permeable membrane with fluid on side 1 above and fluid on side 2 below. Letter A points to an aquaporin, a channel protein that lets water cross. Solute dots cannot cross. Side 1 holds 4 solute dots and side 2 holds 10 solute dots. fluid on side 1 fluid on side 2 aquaporin water A selectively permeable membrane Solute dots cannot cross. - Facilitated diffusion: the cell spends energy to open the aquaporin
- Osmosis: movement of water that the cell powers with its energy
- Osmosis: passive movement of water toward the side with less solute
- Osmosis: passive water movement toward the side with more solute
Solute dots cannot cross either membrane. Each side is the same size. Tala reads two lab chambers, each split by a membrane that passes water but not solute. In which chamber does water move on net, and which way?
Lab drawing (model, not to scale). Two chambers, each split by a selectively permeable membrane with side A above and side B below. Chamber 1: side A holds 4 solute dots and side B holds 9. Chamber 2: side A holds 6 solute dots and side B holds 6. Solute dots cannot cross either membrane. Each side is the same size. Lab drawing (model, not to scale). Two chambers, each split by a selectively permeable membrane with side A above and side B below. Chamber 1: side A holds 4 solute dots and side B holds 9. Chamber 2: side A holds 6 solute dots and side B holds 6. Solute dots cannot cross either membrane. Each side is the same size. Lab drawing (model, not to scale) Chamber 1 side A side B Chamber 2 side A side B selectively permeable membrane - Both chambers, from side A to side B, since water moves in one direction
- Chamber 2, from side A to side B, because its sides are equal
- Chamber 1, from side B to side A, where solute is lower
- Chamber 1, from side A to side B, where solute is higher
Zee compares two U-tubes with a membrane that passes water but not sugar. Which tube shows the faster net movement of water, and why?
Lab drawing (model, not to scale). Two U-tubes, each with a selectively permeable membrane across the bottom. Tube 1: the left arm holds 3 sugar dots and the right arm holds 5. Tube 2: the left arm holds 1 sugar dot and the right arm holds 9. The arms are the same size and temperature. Lab drawing (model, not to scale). Two U-tubes, each with a selectively permeable membrane across the bottom. Tube 1: the left arm holds 3 sugar dots and the right arm holds 5. Tube 2: the left arm holds 1 sugar dot and the right arm holds 9. The arms are the same size and temperature. Lab drawing (model, not to scale) Tube 1 Tube 2 selectively permeable membrane 3 sugar dots 5 sugar dots 1 sugar dot 9 sugar dots same size and temperature - Tube 2, because its arms differ more in sugar, so water moves faster
- Tube 2, because the membrane spends more energy on a bigger difference
- Tube 1, because its arms are closer to equal, so water moves faster
- The tubes match, because water moves toward the right arm in both