Enzymes: how they speed reactions, and what pH and temperature do
Grade 9 Science Worksheet
Circle the best answer.
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Model drawing, not to scale. Enzyme E has a notch, its active site, shaped to hold a round bead joined to a square bead. Molecule M1: a round bead joined to a square bead. Molecule M2: a round bead joined to a triangle bead. Molecule M3: a square bead joined to a square bead.
Enzyme E has an active site shaped for a round bead joined to a square bead. Which molecule can Ari’s enzyme E work on?
- Molecule M3, because enzymes work on any molecule they touch
- Molecule M2, because it shares a round bead with the active site
- Molecule M2, because a triangle bead is a better fit than a square
- Molecule M1, because its beads match the active site
Two digestive enzymes (real data, from OpenStax Biology)
enzyme works in substrate it breaks down pepsin stomach protein salivary amylase mouth starch Pepsin works on proteins in the stomach. Bo wonders what pepsin does if it meets a starch molecule. Use the table.
- Pepsin breaks the starch down, because enzymes work on any food molecule
- Nothing happens, because starch does not fit pepsin’s active site
- Pepsin splits the starch, and then pepsin is used up
- Pepsin breaks the starch down, because stomach acid supplies the energy
Model drawing, not to scale. Esme’s enzyme G holds three beads in a row: round, square, round. Which substrate fits it? Use the drawing.
- Molecule N1, because two matching beads is close enough
- Molecule N2, because its three beads match in order
- Molecule N4, because the enzyme reshapes to fit anything
- Molecule N3, because it has the same number of beads
The model enzyme in the graph works best near pH 2. Kira changes the mixture to pH 7. Predict the activity and choose the reason.
- Near 100, because the added liquid supplies extra energy to the reaction
- Near 0, because pH 7 uses up the enzyme
- Near 100, because a neutral pH is gentler on most enzymes
- Near 0, because pH 7 is far outside the enzyme’s best range
A mixture holds a fixed amount of enzyme. Luz raises the substrate from 5 to 7. Using the graph, predict the rate and the reason.
- It stays near 90, because the active sites are full
- It rises, because the extra substrate brings extra energy
- It falls, because the extra substrate uses up the enzyme
- It rises, because each extra substrate adds to the rate
Milo reads an energy diagram for one reaction with and without an enzyme. What is the activation energy with the enzyme?
- 20 energy units
- 60 energy units
- 95 energy units
- 55 energy units
Using Noah’s energy diagram, by how much does the enzyme lower the activation energy?
- 15 energy units
- 35 energy units
- 50 energy units
- 45 energy units
Which statement describes what the enzyme does to Pia’s energy diagram?
- It raises the end from 20 to 50, adding energy to the products
- It raises the start from 50 to 75, so the reaction begins sooner
- It lowers the peak from 110 to 75; the start and end stay at 50 and 20
- It lowers the peak from 110 to 75, and then the enzyme is used up
Tala knows the stomach’s pH is between 1.5 and 2.5. Which model enzyme, P or Q, suits the stomach, and why?
- Enzyme P, because it is most active near pH 2
- Enzyme P, because the acid supplies the energy it needs
- Enzyme P, but stomach acid will use it up
- Enzyme Q, because its ideal pH is close to neutral, like water
Where two enzymes work (real data, from OpenStax Biology and OpenStax Anatomy and Physiology)
enzyme works in pH there pepsin stomach 1.5 to 2.5 salivary amylase mouth 6.35 to 6.85 Zee reads that salivary amylase has a working range of pH 6.35 to 6.85. Use the table. What happens to it in the stomach?
- It starts working on protein, because enzymes fit any substrate
- It stops working, because the stomach’s acid is far from the pH it needs
- It keeps working the same, because acid does not affect enzymes
- It speeds up, because the acid adds energy