Enzymes: how they speed reactions, and what pH and temperature do
Grade 9 Science Worksheet - Building Confidence

Circle the best answer.
Read this first
Use this
Enzyme drawing (model, not to scale).
Ari studies an enzyme drawing. Letter A points to the notch on top where the small molecule sits. What is A called?
- The active site
- The product
- A notch that fits any molecule
- The substrate
Esme looks at the right side of the drawing, after the reaction. Letter D points to the separate beads. What are they called?
Use the picture in question 1.
- The enzyme, used up
- The cofactors
- The products
- The substrates
Jun’s class compares two energy diagrams of one reaction, one with an enzyme and one without. Which curve shows the reaction with the enzyme?
Energy diagram (model data, not to scale). Reaction progress across. Energy (energy units) up, 0 to 120, a mark every 20. Every curve starts at 40 on the left and ends at 20 on the right. Curve 1 climbs to a peak at 100. Curve 2 climbs to a peak at 60. Energy diagram (model data, not to scale). Reaction progress across. Energy (energy units) up, 0 to 120, a mark every 20. Every curve starts at 40 on the left and ends at 20 on the right. Curve 1 climbs to a peak at 100. Curve 2 climbs to a peak at 60. 0 20 40 60 80 100 120 Reaction progress Energy (energy units) Curve 1 Curve 2 Not to scale. - Curve 1, because the enzyme makes the reaction harder to start
- Curve 1, because the enzyme gives the reaction extra energy to climb higher
- Curve 2, because the enzyme is used up on the way up
- Curve 2, because the enzyme lowers the energy needed to start
Luz reads two energy curves for one reaction: X without an enzyme and Y with one. Which statement about them is true?
Energy diagram (model data, not to scale). Reaction progress across. Energy (energy units) up, 0 to 120, a mark every 20. Every curve starts at 50 on the left and ends at 30 on the right. Curve X climbs to a peak at 100. Curve Y climbs to a peak at 70. Energy diagram (model data, not to scale). Reaction progress across. Energy (energy units) up, 0 to 120, a mark every 20. Every curve starts at 50 on the left and ends at 30 on the right. Curve X climbs to a peak at 100. Curve Y climbs to a peak at 70. 0 20 40 60 80 100 120 Reaction progress Energy (energy units) X Y Not to scale. - Curve Y stops at its peak, so the enzyme is used up
- Curve Y starts higher, so the enzyme brings extra energy
- Curve X ends lower, so it releases more energy
- Both curves start and end at the same energy; the peaks differ
Enzyme drawing (model, not to scale). Milo reads that an enzyme is a catalyst. Which sentence tells what a catalyst does?
Enzyme drawing (model, not to scale). On the left, a large rounded shape with a notch on top; a small molecule of one round bead joined to one square bead sits in the notch. On the right, after the reaction, the same large rounded shape has its notch empty, and the round bead and the square bead have drifted apart. No letters are shown. Enzyme drawing (model, not to scale). On the left, a large rounded shape with a notch on top; a small molecule of one round bead joined to one square bead sits in the notch. On the right, after the reaction, the same large rounded shape has its notch empty, and the round bead and the square bead have drifted apart. No letters are shown. - It speeds up a reaction and turns into a product
- It speeds up a reaction and is not used up
- It is a tiny living helper that works on its own
- It supplies the energy that the reaction releases
Energy diagram (model data, not to scale). Noah reads an energy diagram that has a bracket labeled Z from the starting energy up to the peak. What does Z measure?
Energy diagram (model data, not to scale). Reaction progress across. Energy (energy units) up, 0 to 120, a mark every 20. Every curve starts at 30 on the left and ends at 10 on the right. The curve climbs to a peak at 90. A bracket labeled Z runs from the starting energy up to the top of the peak. Energy diagram (model data, not to scale). Reaction progress across. Energy (energy units) up, 0 to 120, a mark every 20. Every curve starts at 30 on the left and ends at 10 on the right. The curve climbs to a peak at 90. A bracket labeled Z runs from the starting energy up to the top of the peak. Energy (energy units) 0 20 40 60 80 100 120 30 90 Z Reaction progress - The activation energy
- The energy the reaction releases
- The energy an enzyme uses up
- The energy an enzyme adds
Enzyme drawing (model, not to scale). Pia reads that heat can denature an enzyme. What does denature mean here?
Enzyme drawing (model, not to scale). On the left, a large rounded shape with a notch on top; a small molecule of one round bead joined to one square bead sits in the notch. On the right, after the reaction, the same large rounded shape has its notch empty, and the round bead and the square bead have drifted apart. No letters are shown. Enzyme drawing (model, not to scale). On the left, a large rounded shape with a notch on top; a small molecule of one round bead joined to one square bead sits in the notch. On the right, after the reaction, the same large rounded shape has its notch empty, and the round bead and the square bead have drifted apart. No letters are shown. - The enzyme speeds up because it holds more energy from the heat
- The enzyme’s shape changes, so the substrate no longer fits
- The enzyme, a tiny living thing, stops moving in the heat
- The enzyme splits into products and is used up by the heat
Ren graphs a model enzyme’s activity at different temperatures. At which temperature is the activity highest?
Activity-versus-temperature graph for a model enzyme (model data). Temperature (°C) across, 0 to 70, a mark every 10. Enzyme activity (% of the highest rate) up, 0 to 100, a mark every 20. The line joins (0 °C, 5), (10 °C, 15), (20 °C, 35), (30 °C, 65), (40 °C, 100), (50 °C, 40), (60 °C, 5), (70 °C, 0) with straight pieces. Activity-versus-temperature graph for a model enzyme (model data). Temperature (°C) across, 0 to 70, a mark every 10. Enzyme activity (% of the highest rate) up, 0 to 100, a mark every 20. The line joins (0 °C, 5), (10 °C, 15), (20 °C, 35), (30 °C, 65), (40 °C, 100), (50 °C, 40), (60 °C, 5), (70 °C, 0) with straight pieces. Activity-versus-temperature graph for a model enzyme (model data) 0 10 20 30 40 50 60 70 0 20 40 60 80 100 Temperature (degC) Enzyme activity (% of the highest rate) - 40 °C
- 70 °C, the hottest tested
- 30 °C
- 20 °C
Use Tala’s graph of a second model enzyme. What is its activity at 40 °C?
Activity-versus-temperature graph for a second model enzyme (model data). Temperature (°C) across, 0 to 70, a mark every 10. Enzyme activity (% of the highest rate) up, 0 to 100, a mark every 20. The line joins (0 °C, 10), (10 °C, 30), (20 °C, 60), (30 °C, 100), (40 °C, 70), (50 °C, 20), (60 °C, 0), (70 °C, 0) with straight pieces. Activity-versus-temperature graph for a second model enzyme (model data). Temperature (°C) across, 0 to 70, a mark every 10. Enzyme activity (% of the highest rate) up, 0 to 100, a mark every 20. The line joins (0 °C, 10), (10 °C, 30), (20 °C, 60), (30 °C, 100), (40 °C, 70), (50 °C, 20), (60 °C, 0), (70 °C, 0) with straight pieces. Activity-versus-temperature graph for a second model enzyme (model data) 0 10 20 30 40 50 60 70 0 20 40 60 80 100 Temperature (degC) Enzyme activity (% of the highest rate) - 100
- 70
- 60
- 20
Zee reads a third model enzyme’s graph. What happens to its activity as the temperature rises from 50 °C to 70 °C?
Activity-versus-temperature graph for a third model enzyme (model data). Temperature (°C) across, 0 to 70, a mark every 10. Enzyme activity (% of the highest rate) up, 0 to 100, a mark every 20. The line joins (0 °C, 0), (10 °C, 5), (20 °C, 15), (30 °C, 35), (40 °C, 70), (50 °C, 100), (60 °C, 40), (70 °C, 0) with straight pieces. Activity-versus-temperature graph for a third model enzyme (model data). Temperature (°C) across, 0 to 70, a mark every 10. Enzyme activity (% of the highest rate) up, 0 to 100, a mark every 20. The line joins (0 °C, 0), (10 °C, 5), (20 °C, 15), (30 °C, 35), (40 °C, 70), (50 °C, 100), (60 °C, 40), (70 °C, 0) with straight pieces. Activity-versus-temperature graph for a third model enzyme (model data) 0 10 20 30 40 50 60 70 0 20 40 60 80 100 Temperature (degC) Enzyme activity (% of the highest rate) - It stays at 100, because the enzyme adjusts like a living thing
- It keeps rising, because more heat means more speed
- It falls, because heat changes the enzyme’s shape
- It falls, because the heat uses up the enzyme