Microscopes: parts, types and total magnification
Grade 9 Science Worksheet - Building Confidence

Circle the best answer.
A drawing of a cell is 36 mm long and made at 900×. Ari wants the real cell's length. How long is it?
- 0.04 µm
- 40 µm
- 400 µm
- 32,400 µm
A photo shows a cell 60 mm long. Bo knows the real cell is 30 µm long. What is the magnification?
- 2×
- 2,000×
- 200×
- 1,800,000×
Read this first
Red blood cell on a photo (example data)
item length on the photo (mm) real length (µm) scale bar 25 5 red blood cell 40 ? Esme uses the photo's scale bar to size a red blood cell. How wide is the real cell?
- 8 mm
- 8 µm
- 3.1 µm
- 200 µm
1 mm = 1,000 µm. Jun reads two real sizes. How many red blood cells fit side by side across the pinhead?
- 16,000 cells
- 25 cells
- 250 cells
- 0.25 cells
Light microscope resolution (real data, from OpenStax Biology 2e)
measure value resolution about 200 nanometers 1 µm = 1,000 nm.
Kira views a rod-shaped bacterium 0.5 µm long. Can a light microscope show its two ends as separate points?
- No: 0.5 is smaller than 200, so it is too small
- Yes: 0.5 µm is 500 nm, which is more than 200 nm
- No, but a stronger eyepiece would separate them
- An electron microscope could separate them, but a light one could not
Two dots on a slide are 0.1 µm apart. Luz asks whether the same light microscope shows them as two dots. What is the answer?
- No: 0.1 µm is 100 nm, closer than the 200 nm limit
- Yes: 0.1 µm is bigger than 200 nm
- Yes: 100 is less than 200, so they are easy to separate
- Yes, if the total magnification is high enough
Field of view (example data)
total magnification field diameter (mm) 100× 1.8 400× 0.45 The field of view is 1.8 mm wide at 100×. Milo switches to 400×. How wide is the field now?
- 7.2 mm
- 0.9 mm
- 0.45 mm
- 0.045 mm
Two kinds of microscope (real data, from OpenStax Biology 2e)
microscope magnification resolution living cells? light (college lab) about 400 times about 200 nanometers yes electron about 100,000 times 50 picometers no 1 nm = 1,000 pm.
Ren compares resolutions. How many times finer is the electron microscope's resolution than the light microscope's?
- About 4 times finer
- About 4,000,000 times finer
- About 4,000 times finer
- About 400 times finer
Two structures are 100 nm apart. Tala wants to see them as two. Which microscope works?
- Either one, since 100 nm is a large gap
- An electron microscope, since 50 pm is far finer than 100 nm
- A light microscope, because it can look at living cells
- A light microscope at its highest power
Two electron microscopes (facts from OpenStax Biology 2e)
kind how the beam works what it shows scanning moves back and forth across the surface surface details transmission passes through a thin slice internal structures Zee wants to see the inside of a cell's nucleus and mitochondria. Which microscope works?
- A transmission electron microscope, since its beam passes through a thin slice
- A light microscope, since it shows inside structures finest
- A scanning electron microscope, since it moves across the specimen
- A scanning electron microscope, since electron beams show the inside too