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9th grade The structure of DNA worksheet

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The structure of DNA

Grade 9 Science Worksheet - Building Confidence

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Circle the best answer.

  1. Ari labels a paper model of DNA. Arrow W points to the long outer line of the top strand. What is W?

    • A line of hydrogen bonds holding the two strands together
    • A row of bases, because the bases form the outer lines
    • The sugar-phosphate backbone, a chain of sugars and phosphates
    • A stack of base pairs, one resting on the next one
  2. Ladder (model, not to scale). Bo keeps labeling the paper model. Arrow X points to one short crosspiece between the two strands. What is X?

    • A piece of the backbone, because the sugars and phosphates form the rungs
    • A base pair, two bases joined by hydrogen bonds
    • A gene, a long stretch that holds the plan for a protein
    • A nucleotide, a sugar with a phosphate and a base
  3. Jun fills in a paper ladder. Which base goes in the blank, directly under adenine (A)?

    • Guanine (G), because both bases have double rings
    • Cytosine (C), the partner of guanine
    • Adenine (A), because matching letters go together
    • Thymine (T), the partner of adenine
  4. Kira fills in a second paper ladder. Which base goes in the blank, directly under cytosine (C)?

    • Thymine (T), because both bases have single rings
    • Adenine (A), the partner of thymine
    • Guanine (G), the partner of cytosine
    • Cytosine (C), because matching letters go together
  5. Ladder (model, not to scale). Luz completes the bottom strand under A, C, G. Which three bases fill the blanks, left to right?

    • T, G, C
    • A, C, G
    • G, T, A
    • C, G, T
  6. Two drawings (not to scale). Milo compares two drawings of the dotted hydrogen bonds between paired bases. Which case is correct?

    • Case Q, because A–T has 3 bonds and C–G has 2
    • Case Q, because a pair with a double ring has 3 bonds
    • Case P, because the two kinds of pair have the same number of bonds
    • Case P, because A–T has 2 bonds and C–G has 3
  7. “Two drawings (not to scale). Columns:” Pia compares two drawings of a short stretch of DNA. Which case puts the parts in the right places?

    • Case B, because the phosphates join the two strands in the middle
    • Case A, because the bases need to be outside to pair up
    • Case A, because the bases form the long outer lines
    • Case B, because the bases meet inside the chains
  8. Ren reads a history card. According to the card, who made the X-ray photo labeled 51?

    • Raymond Gosling, working alone
    • James Watson and Francis Crick, at Cambridge
    • Maurice Wilkins, who received the 1962 prize
    • Rosalind Franklin, with Raymond Gosling
  9. Tala reads the same history card. Where did Watson and Crick get X-ray data for their double-helix model?

    Use the picture in question 8.

    • From an X-ray photo they took themselves at Cambridge
    • From the 1962 prize paper, written after the model
    • From Franklin's X-ray work at King's College London
    • They used no X-ray data and built the model from parts
  10. Card of base-pair facts (Data: OpenStax Biology 2e). Columns: base pair, hydrogen bonds. A–T: 2. C–G: 3. Zee reads a card of facts about base pairs. How many hydrogen bonds join a C–G pair?

    • 2 hydrogen bonds
    • 1 hydrogen bond
    • 5 hydrogen bonds
    • 3 hydrogen bonds
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9th grade The structure of DNA worksheet – printable
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About this worksheet

The structure of DNA: nucleotides, base pairs and the double helix. Every version has 10 questions, and the answer key prints on the second page.

What your child will learn

  • I can say a nucleotide has three parts, a five-carbon sugar (deoxyribose in DNA), a phosphate group and a nitrogen-containing base, and point to one on a drawing.
  • I can say the sugar-phosphate backbone forms the two outer rails, and the bases stack inside, facing the other strand.
  • I can name the four bases (A, T, G, C), say that A and G are purines with a double ring and C and T are pyrimidines with a single ring, and say why every rung joins one of each.
  • I can write the partner of a strand: A with T and C with G.
  • I can say that A–T pairs are held by 2 hydrogen bonds and C–G pairs by 3, and count the bonds in a stretch.
  • I can say the two strands run in opposite directions (antiparallel), so the 5′ end of one lies against the 3′ end of the other, and write a partner strand from its own 5′ end.

Standard: HS-LS1-1, HS-LS3-1

Words to know

nucleotide: One unit of DNA: a sugar, a phosphate and a base

deoxyribose: The five-carbon sugar in DNA

phosphate: The part of a nucleotide that links to the sugar of the next one

base: The lettered part of a nucleotide: A, T, G or C

sugar-phosphate backbone: The outer rail of a strand, made of sugars and phosphates

purine: A base with a double ring: A or G

pyrimidine: A base with a single ring: C or T

base pair: Two bases, one from each strand, joined by hydrogen bonds

Common mistakes

Your child paired a base with the wrong partner. In DNA, A pairs with T and C pairs with G, so a double-ring base always meets a single-ring base. Ask your child to say each pair aloud before choosing.

Your child put the bases on the outside of the ladder. The sugar-phosphate backbone forms the two outer rails, and the bases meet in the middle. Ask your child which part forms the rails.

Your child thought the two strands of DNA run in the same direction. They run in opposite directions, so the 5′ end of one faces the 3′ end of the other. Ask your child to mark the ends on both strands.

Questions parents ask

What is the most common mistake with the structure of dna?

Your child paired a base with the wrong partner. In DNA, A pairs with T and C pairs with G, so a double-ring base always meets a single-ring base. Ask your child to say each pair aloud before choosing.

Are these 9th grade science worksheets free to print?

Yes. All 6 printable versions at each level are free to print, with the answer key, and no account is needed.

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