PencilGo

Grade 9 · Science · Biology

9th grade With and without oxygen: respiration and fermentation worksheet

6 printable versionsChallenge Me10 questions · answer keyFree · no account

Level: Building Confidence · On Level · Challenge Me

When it’s done, scan the sheet with your phone and PencilGo marks it and picks the next one. Create a free account

Version
Print

With and without oxygen: respiration and fermentation

Grade 9 Science Worksheet - Challenge Me

NameDate

Circle the best answer.

  1. Read this first

    Use this

    ATP card (sourced values, per 1 glucose). The cell has no oxygen at first: fermentation, 2 ATP in total. After oxygen is added, pyruvate enters the mitochondrion: aerobic respiration, about 30 ATP in total.

  2. A cell that can ferment or use aerobic respiration has been fermenting 1 glucose. Pia adds oxygen. Predict the ATP total per glucose, and why.

    • It rises from 2 to about 30 ATP, since pyruvate now enters the mitochondrion
    • It rises from 0 to about 30 ATP, since the cell made no ATP before
    • It falls from 2 to 0 ATP, since oxygen would stop glycolysis
    • It stays at 2 ATP, since fermentation and aerobic respiration make the same total
  3. “model, not to scale” A chemical blocks the step that turns NADH into NAD+ in a muscle cell without oxygen. Ren predicts what happens to glycolysis and its ATP, and why.

    • Glycolysis continues unchanged, because just the mitochondrion is affected
    • Glycolysis continues, because glycolysis takes the oxygen it needs from the muscle cell
    • Glycolysis continues at 2 ATP per glucose, because built-up NADH acts as fuel
    • Glycolysis slows to a stop and ATP from glucose stops, because NAD+ is not replaced
  4. ATP card (sourced values)

    pathATP in total per 1 glucose
    Aerobic respirationabout 30
    Fermentation2
  5. Tala's class models a muscle cell given 10 glucose. With oxygen it makes about 300 ATP. Oxygen is removed, so only glycolysis and fermentation remain. Predict the new total.

    • It falls from about 300 to 0 ATP, because ATP needs oxygen
    • It falls from about 300 to 0 ATP, because muscle cells cannot ferment like yeast
    • It stays near 300 ATP, because glycolysis makes most of the cell's ATP
    • It falls from about 300 to 20 ATP
  6. Use this

    Two cards. Mud core card (model data, 4 rows). Columns: depth (cm), oxygen (mg/L). 0, 6. 1, 2. 3, 0. 8, 0. Final electron acceptor card (sourced facts, 4 rows). Columns: final electron acceptor, becomes. Oxygen, water. Sulfate, hydrogen sulfide gas. Iron (ferric), iron (ferrous). Carbon dioxide, methane.

  7. Zee's class reads two cards about wetland mud. Microbes at 8 cm make ATP with an electron transport chain. Which statement fits both cards?

    • Oxygen reads 0, so a chain there must end with another acceptor, such as sulfate
    • Oxygen reads 0, so those microbes make no ATP and release nothing
    • Oxygen reads 6, so those microbes end the chain with oxygen and make water
    • Oxygen reads 0, so those microbes ferment, which is the same as ending a chain with sulfate
  8. Use this

    Two cards. ATP card (sourced values, 1 row). Columns: path, ATP in total per 1 glucose. Fermentation, 2. NAD+ card (sourced facts, 1 row). Columns: stage, NAD+ regenerated per 1 glucose. Fermentation, 2.

  9. A model muscle sample made 50 ATP in total by fermentation alone. Bo uses both cards to find how many glucose were used and how many NAD+ were regenerated.

    • 25 glucose and 25 NAD+
    • About 2 glucose and 4 NAD+
    • 25 glucose and 50 NAD+
    • 25 glucose and 0 NAD+
  10. Use this

    Notebook lines (model data, 4 rows). Columns: line, notebook says. 1, Yeast cells in bread dough ferment sugar and release CO2 gas. 2, Bacteria in yogurt ferment milk sugar into lactic acid. 3, Muscle cells cannot ferment, so only yeast cells use fermentation. 4, Some microbes in wetland mud use sulfate instead of oxygen.

  11. Jun's notebook lists four lines about microbes and cells. One line has a flaw. Which line has the flaw?

    • Line 3: muscle cells and some bacteria also ferment, so yeast are not the only ones
    • Line 2: yogurt bacteria need oxygen to ferment the milk sugar
    • Line 1: yeast fermentation makes lactic acid, not a gas
    • Line 4: microbes in mud make no ATP because they lack oxygen
  12. Kira's class concludes that sugar makes yeast release gas. Read about only. Kira's class reads about the dough test and concludes that sugar makes yeast release gas. What is the flaw in the test?

    • Sugar and temperature both differ, so sugar cannot be named as the cause
    • Cup B should have had more yeast than Cup A to be fair
    • Height is the wrong measure, since ATP counts are what show fermentation
    • Cup B needed oxygen added, since fermentation cannot run without it
  13. ATP card (sourced values)

    pathATP in total per 1 glucose
    Aerobic respirationabout 30
    Fermentation2
  14. Luz's classmate says sprinting muscle cells switch to fermentation because it makes more ATP than aerobic respiration. Do you agree?

    • Agree: fermentation nets about 30 ATP per glucose, so it is the better choice
    • Disagree: muscle cells do not ferment; yeast cells do
    • Disagree: fermentation makes about 2 ATP per glucose against about 30, but it keeps glycolysis going when oxygen is short
    • Disagree: without oxygen, muscle cells make no ATP
  15. Final electron acceptor card (sourced facts)

    final electron acceptorbecomes
    Oxygenwater
    Sulfatehydrogen sulfide gas
    Iron (ferric)iron (ferrous)
    Carbon dioxidemethane
  16. Milo's classmate says glycolysis cannot continue without oxygen, so microbes in oxygen-free mud make no ATP. Do you agree?

    • Agree: glycolysis uses oxygen directly, so it stops in mud
    • Disagree: regenerating NAD+ lets glycolysis continue, and some microbes end a chain with sulfate or iron
    • Disagree: NADH built up in the cell supplies the ATP
    • Disagree: fermentation needs oxygen, which the mud supplies
  17. Noah's classmate says mud microbes that end an electron transport chain with sulfate are fermenting. Do you agree?

    • Agree: any process that runs without oxygen is fermentation
    • Disagree: sulfate users make no ATP, so neither process applies
    • Disagree: they use the oxygen in sulfate for aerobic respiration
    • Disagree: they run an electron transport chain ending with sulfate, which is anaerobic respiration
PencilGopencilgo.com

9th grade With and without oxygen: respiration and fermentation worksheet – printable
Print: 123456

About this worksheet

With and without oxygen: respiration and fermentation. Every version has 10 questions, and the answer key prints on the second page.

What your child will learn

  • I can say glycolysis runs first in every case (1 glucose becomes 2 pyruvate, a net of 2 ATP and 2 NADH, in the cytoplasm, with no oxygen used directly), and that oxygen decides what comes next.
  • I can name the two branches. With oxygen, pyruvate enters the mitochondrion and oxygen is the final electron acceptor (aerobic respiration, about 30 ATP in total per glucose). Without oxygen, glycolysis can go on only if NADH is turned back into NAD+, which is the job of fermentation.
  • I can say fermentation uses no oxygen, has no electron transport chain, and adds no ATP beyond the 2 from glycolysis, so a fermenting cell makes 2 ATP per glucose, about one fifteenth of the aerobic total.
  • I can follow the NAD+ round: glycolysis turns NAD+ into NADH, fermentation turns it back, so the supply is reused (2 NAD+ regenerated per glucose, as in pyruvate + NADH → lactate + NAD+).
  • I can place lactic acid fermentation: in muscle cells short of oxygen in a hard sprint, and in yogurt bacteria, where the lactic acid sours the milk and clumps its proteins. I can say yeast fermentation in dough releases carbon dioxide, which makes the dough rise, and that muscle cells and bacteria ferment too, so it is not only yeast.
  • I can tell fermentation from anaerobic respiration: some bacteria and archaea run an electron transport chain that ends with a molecule other than oxygen (sulfate, iron or carbon dioxide), so a chain, not just the lack of oxygen, marks anaerobic respiration.

Standard: HS-LS1-7, HS-LS2-3

Words to know

aerobic: Using oxygen; aerobic respiration ends with oxygen as the final electron acceptor

anaerobic: Without oxygen; glycolysis, fermentation and anaerobic respiration run with no oxygen

glycolysis: The first stage: glucose is split into two pyruvate in the cytoplasm

pyruvate: A three-carbon molecule made when glucose is split

NAD+ and NADH: An electron carrier: NAD+ picks up electrons to become NADH, and gives them up again

fermentation: Turning NADH back into NAD+ with an organic molecule, so glycolysis can go on

lactic acid fermentation: Pyruvate + NADH → lactate + NAD+; in muscle cells and some bacteria

lactate (lactic acid): The three-carbon product of lactic acid fermentation

Common mistakes

Your child thought fermentation makes lots of ATP. Fermentation adds none beyond the 2 ATP from glycolysis, while aerobic respiration makes about 30 per glucose. Ask your child which path makes more ATP, and how many.

Your child thought a cell without oxygen makes no ATP. Glycolysis plus fermentation still makes 2 ATP per glucose, and some microbes run an electron transport chain on other molecules. Ask your child what a muscle cell does when oxygen runs short.

Your child thought fermentation needs oxygen. Fermentation runs in the cytoplasm with no oxygen at all. Ask your child which branch of the diagram uses oxygen.

Questions parents ask

What is the most common mistake with with and without oxygen: respiration and fermentation?

Your child thought fermentation makes lots of ATP. Fermentation adds none beyond the 2 ATP from glycolysis, while aerobic respiration makes about 30 per glucose. Ask your child which path makes more ATP, and how many.

Are these 9th grade science worksheets free to print?

Yes. All 6 printable versions at each level are free to print, with the answer key, and no account is needed.

More biology

See all Biology

Every sheet you scan goes into your child’s record: dated, marked and ready for the year-end portfolio. Homeschool records