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9th grade With and without oxygen: respiration and fermentation worksheet

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With and without oxygen: respiration and fermentation

Grade 9 Science Worksheet - Building Confidence

NameDate

Circle the best answer.

  1. Read this first

    Use this

    Flow diagram (model, not to scale), drawn across a cell. Input box on the left: 1 glucose. Stage 1, in the cytoplasm: glycolysis makes 2 pyruvate. The path then splits. Branch A, oxygen absent: fermentation in the cytoplasm. Branch B, oxygen present: pyruvate enters the mitochondrion.

  2. Ari reads a flow diagram of a cell with no oxygen. After glycolysis, which branch runs?

    • Branch B, because fermentation needs oxygen to run
    • Both branches, because pyruvate has to use each one
    • Neither branch, because a cell with no oxygen makes no ATP
    • Branch A, because fermentation does not need oxygen
  3. Use this

    Flow diagram (model, not to scale), drawn across a cell. Input box on the left: 1 glucose. Stage A turns 1 glucose into 2 pyruvate, with a net of 2 ATP. Then branch 1 (oxygen present) and branch 2 (oxygen absent) both start from the pyruvate.

  4. Esme sees stage A at the start of both branches. In stage A, 1 glucose becomes 2 pyruvate. Name stage A and its place.

    • Fermentation, in the cytoplasm
    • Fermentation, in the mitochondrion
    • Glycolysis, in the cytoplasm
    • Glycolysis, in the mitochondrion
  5. Jun reads a reaction in a muscle cell low on oxygen: pyruvate + NADH → A + NAD+. What goes in box A?

    • Lactate, also called lactic acid
    • ATP, made by the fermentation step
    • NADH, made again from the NAD+
    • Carbon dioxide, released as a gas
  6. Flow diagram (model, not to scale). No wavy arrow is drawn. Kira reads about yeast in bread dough. The yeast ferment sugar and release gas A, which makes the dough rise. Name gas A.

    • Oxygen gas (O2) from the air
    • Lactic acid, a liquid from the sugar
    • Carbon dioxide gas (CO2)
    • Hydrogen gas (H2) from the sugar
  7. Use this

    Flow diagram (model, not to scale), drawn in a bacterium in warm milk. A, a substance that makes the milk sour and thick. No wavy arrow is drawn.

  8. Bacteria in warm milk ferment the milk sugar. Luz asks what the bacteria make that sours and thickens the milk.

    • Oxygen, which the bacteria need to ferment
    • Nothing, because bacteria cannot ferment; yeast do
    • Lactic acid, made from the milk sugar
    • Carbon dioxide gas, which makes the milk rise
  9. The words are aerobic respiration. Four meanings are listed in the choices. Milo matches the words aerobic respiration to their meaning. Which meaning is right?

    • Respiration that nets about 2 ATP per glucose in total
    • Respiration that makes ATP without using any oxygen
    • A way to turn NADH back into NAD+ with pyruvate
    • Respiration that uses oxygen as the final electron acceptor
  10. The word is anaerobic, on a card about mud. Four meanings are listed in the choices. Pia sees the word anaerobic on a card about mud. What does anaerobic describe?

    • A process that cannot make any ATP
    • A process found in yeast cells alone
    • A process that runs without oxygen
    • A process that needs oxygen to get started
  11. ATP card (sourced values)

    pathATP in total per 1 glucose
    Aerobic respirationabout 30
    Fermentation2
  12. Ren reads the ATP card. How many ATP does fermentation make in total from 1 glucose?

    • about 30 ATP
    • 4 ATP
    • 2 ATP
    • 0 ATP
  13. Cell card (model data)

    celloxygenATP in total
    Cell Ppresentabout 30
    Cell Qabsent2
  14. Tala reads a card of two cells that each start with 1 glucose. Which cell makes more ATP in total?

    • Cell P, with about 30 ATP
    • Neither, because the two cells make the same
    • Cell Q, because fermentation makes more ATP
    • Cell P, with about 2 ATP, since oxygen cells make less
  15. Use this

    Flow diagram (model, not to scale), drawn in the cytoplasm. Input box on the left: 1 glucose. Output boxes on the right: 2 pyruvate; net 2 ATP; B NADH, where B is a blank number.

  16. Zee reads the glycolysis box. How many NADH does glycolysis make from 1 glucose?

    • 0 NADH, because NADH is made in the mitochondrion
    • 2 NADH, one for each pyruvate
    • 4 NADH, two for each pyruvate
    • 1 NADH, one for each glucose
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9th grade With and without oxygen: respiration and fermentation worksheet – printable
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About this worksheet

With and without oxygen: respiration and fermentation. Every version has 10 questions, and the answer key prints on the second page.

What your child will learn

  • I can say glycolysis runs first in every case (1 glucose becomes 2 pyruvate, a net of 2 ATP and 2 NADH, in the cytoplasm, with no oxygen used directly), and that oxygen decides what comes next.
  • I can name the two branches. With oxygen, pyruvate enters the mitochondrion and oxygen is the final electron acceptor (aerobic respiration, about 30 ATP in total per glucose). Without oxygen, glycolysis can go on only if NADH is turned back into NAD+, which is the job of fermentation.
  • I can say fermentation uses no oxygen, has no electron transport chain, and adds no ATP beyond the 2 from glycolysis, so a fermenting cell makes 2 ATP per glucose, about one fifteenth of the aerobic total.
  • I can follow the NAD+ round: glycolysis turns NAD+ into NADH, fermentation turns it back, so the supply is reused (2 NAD+ regenerated per glucose, as in pyruvate + NADH → lactate + NAD+).
  • I can place lactic acid fermentation: in muscle cells short of oxygen in a hard sprint, and in yogurt bacteria, where the lactic acid sours the milk and clumps its proteins. I can say yeast fermentation in dough releases carbon dioxide, which makes the dough rise, and that muscle cells and bacteria ferment too, so it is not only yeast.
  • I can tell fermentation from anaerobic respiration: some bacteria and archaea run an electron transport chain that ends with a molecule other than oxygen (sulfate, iron or carbon dioxide), so a chain, not just the lack of oxygen, marks anaerobic respiration.

Standard: HS-LS1-7, HS-LS2-3

Words to know

aerobic: Using oxygen; aerobic respiration ends with oxygen as the final electron acceptor

anaerobic: Without oxygen; glycolysis, fermentation and anaerobic respiration run with no oxygen

glycolysis: The first stage: glucose is split into two pyruvate in the cytoplasm

pyruvate: A three-carbon molecule made when glucose is split

NAD+ and NADH: An electron carrier: NAD+ picks up electrons to become NADH, and gives them up again

fermentation: Turning NADH back into NAD+ with an organic molecule, so glycolysis can go on

lactic acid fermentation: Pyruvate + NADH → lactate + NAD+; in muscle cells and some bacteria

lactate (lactic acid): The three-carbon product of lactic acid fermentation

Common mistakes

Your child thought fermentation makes lots of ATP. Fermentation adds none beyond the 2 ATP from glycolysis, while aerobic respiration makes about 30 per glucose. Ask your child which path makes more ATP, and how many.

Your child thought a cell without oxygen makes no ATP. Glycolysis plus fermentation still makes 2 ATP per glucose, and some microbes run an electron transport chain on other molecules. Ask your child what a muscle cell does when oxygen runs short.

Your child thought fermentation needs oxygen. Fermentation runs in the cytoplasm with no oxygen at all. Ask your child which branch of the diagram uses oxygen.

Questions parents ask

What is the most common mistake with with and without oxygen: respiration and fermentation?

Your child thought fermentation makes lots of ATP. Fermentation adds none beyond the 2 ATP from glycolysis, while aerobic respiration makes about 30 per glucose. Ask your child which path makes more ATP, and how many.

Are these 9th grade science worksheets free to print?

Yes. All 6 printable versions at each level are free to print, with the answer key, and no account is needed.

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