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9th grade Enzymes: how they speed reactions, and what pH and temperature do worksheet

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Enzymes: how they speed reactions, and what pH and temperature do

Grade 9 Science Worksheet - Building Confidence

NameDate

Circle the best answer.

  1. Read this first

    Use this

    Enzyme drawing (model, not to scale).

  2. On Bo’s enzyme drawing, letter B points to the joined beads that the enzyme binds. What is B called?

    • The product
    • Any molecule the enzyme meets
    • The substrate
    • The active site
  3. Esme looks at the right side of the drawing, after the reaction. Letter D points to the separate beads. What are they called?

    Use the picture in question 1.

    • The cofactors
    • The enzyme, used up
    • The products
    • The substrates
  4. Jun’s class compares two energy diagrams of one reaction, one with an enzyme and one without. Which curve shows the reaction with the enzyme?

    • Curve 2, because the enzyme lowers the energy needed to start
    • Curve 2, because the enzyme is used up on the way up
    • Curve 1, because the enzyme gives the reaction extra energy to climb higher
    • Curve 1, because the enzyme makes the reaction harder to start
  5. Kira compares curves A and B for one reaction. Only one curve has an enzyme. Which curve is it, and why?

    • Curve A, because enzymes make a reaction release more energy
    • Curve A, because its higher peak shows the enzyme adding energy
    • Curve B, because its lower peak needs less energy to start
    • Curve B, because the enzyme is gone before the reaction ends
  6. Luz reads two energy curves for one reaction: X without an enzyme and Y with one. Which statement about them is true?

    • Curve Y stops at its peak, so the enzyme is used up
    • Both curves start and end at the same energy; the peaks differ
    • Curve Y starts higher, so the enzyme brings extra energy
    • Curve X ends lower, so it releases more energy
  7. Use this

    Enzyme drawing (model, not to scale).

  8. Milo reads that an enzyme is a catalyst. Which sentence tells what a catalyst does?

    • It speeds up a reaction and is not used up
    • It is a tiny living helper that works on its own
    • It supplies the energy that the reaction releases
    • It speeds up a reaction and turns into a product
  9. Pia reads that heat can denature an enzyme. What does denature mean here?

    Use the picture in question 6.

    • The enzyme speeds up because it holds more energy from the heat
    • The enzyme, a tiny living thing, stops moving in the heat
    • The enzyme’s shape changes, so the substrate no longer fits
    • The enzyme splits into products and is used up by the heat
  10. Ren graphs a model enzyme’s activity at different temperatures. At which temperature is the activity highest?

    • 20 °C
    • 70 °C, the hottest tested
    • 40 °C
    • 30 °C
  11. Use Tala’s graph of a second model enzyme. What is its activity at 40 °C?

    • 20
    • 100
    • 60
    • 70
  12. Zee reads a third model enzyme’s graph. What happens to its activity as the temperature rises from 50 °C to 70 °C?

    • It stays at 100, because the enzyme adjusts like a living thing
    • It keeps rising, because more heat means more speed
    • It falls, because the heat uses up the enzyme
    • It falls, because heat changes the enzyme’s shape
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9th grade Enzymes: how they speed reactions, and what pH and temperature do worksheet – printable
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About this worksheet

Enzymes: how they speed reactions, and what temperature and pH do. Every version has 10 questions, and the answer key prints on the second page.

What your child will learn

  • I can name the parts of an enzyme drawing: enzyme, active site, substrate and products.
  • I can say what a catalyst does: it speeds up a reaction by lowering the activation energy, and it comes out unchanged, so it is reused.
  • I can read an energy diagram with and without an enzyme and work out the activation energy from the peak and the start.
  • I can match a substrate to an active site by shape, and explain why one enzyme does not work on every molecule.
  • I can read an activity graph (temperature, pH, substrate amount) and predict what a change does. Heat raises the rate up to a best temperature, then changes the enzyme's shape and the activity drops.
  • I can read a read-about enzyme investigation (catalase and hydrogen peroxide, run by a teacher) and spot a flaw, a missing control or a conclusion the data do not support.

Standard: supports HS-LS1-1

Words to know

enzyme: A protein that speeds up a reaction in a cell

catalyst: Something that speeds up a reaction and is not used up

substrate: The reactant an enzyme binds

active site: The spot on an enzyme where the substrate binds

product: What the reaction makes

activation energy: The energy needed to get a reaction started

denature: Change an enzyme's shape so the substrate no longer fits

optimum: The temperature or pH where an enzyme works best

Common mistakes

Your child thought an enzyme is used up or turned into a product. Ask which part comes out the same at the end, and why one enzyme can work again and again.

Your child thought an enzyme supplies energy to a reaction. An enzyme lowers the hill the reaction must climb. Ask your child to sketch a hill and draw a lower hill with the enzyme.

Your child thought hotter always means faster for an enzyme. Past its best temperature the enzyme changes shape and slows down. Ask where the top of the curve is and what happens after it.

Questions parents ask

What is the most common mistake with enzymes: how they speed reactions, and what ph and temperature do?

Your child thought an enzyme is used up or turned into a product. Ask which part comes out the same at the end, and why one enzyme can work again and again.

Are these 9th grade science worksheets free to print?

Yes. All 6 printable versions at each level are free to print, with the answer key, and no account is needed.

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