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9th grade Transcription: from DNA to messenger RNA worksheet

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Transcription: from DNA to messenger RNA

Grade 9 Science Worksheet - Challenge Me

NameDate

Circle the best answer.

  1. Ari reads a model pre-mRNA. The introns are cut out and the exons are joined. How many bases are in the mature mRNA?

    • 45 bases
    • 33 bases
    • 60 bases
    • 27 bases
  2. Bo reads a second model pre-mRNA. Three bases make one codon. How many codons are in the mature mRNA?

    • 17 codons
    • 19 codons
    • 57 codons
    • 36 codons
  3. Read this first

    Model rate card (model data)

    factvalue
    bases added each second by E. coli RNA polymerase40
    codons in the model mRNA400
    bases in one codon3
  4. Esme reads a rate card. About how many seconds does the polymerase need to build the whole model mRNA?

    • 1,240 seconds
    • 30 seconds
    • 48,000 seconds
    • 10 seconds
  5. Model gene (not to scale). Jun reads a model template. RNA polymerase builds a pre-mRNA from it, and then the intron is cut out. Which mature mRNA is left?

    • UACUGUAAGCUG
    • AUGACAGUAAAGUUCGAC
    • AUGACAUUCGAC
    • ATGACATTCGAC
  6. Luz reads a model template. Which RNA does the polymerase build first, before any splicing?

    • AUGCACCGAUUC
    • UACGUGCAGUUCGCUAAG
    • ATGCACGTCAAGCGATTC
    • AUGCACGUCAAGCGAUUC
  7. Use this

    Notebook on transcription (model data), 4 rows. Columns: line, what it says. 1, RNA polymerase reads the template strand of one gene. 2, The mRNA is built by pairing bases with the template. 3, Compared with the coding strand, the mRNA has U where the DNA has T. 4, The mRNA has the same letters as the template strand.

  8. Milo checks a classmate’s notebook on transcription. Exactly one line is wrong. Which line, and why?

    • Line 1, because both strands of a gene are read
    • Line 2, because mRNA is built from the coding strand
    • Line 3, because RNA keeps T like DNA
    • Line 4, because the mRNA pairs with the template
  9. Notebook on a plant cell’s mRNA (model data)

    linewhat it says
    1Transcription of a gene takes place in the nucleus
    2A cap is added at the 5′ end and a poly-A tail at the 3′ end
    3Introns are cut out and the exons are joined
    4The finished mRNA keeps its introns when it leaves the nucleus
  10. Noah checks a classmate’s notebook on a plant cell’s mRNA. Exactly one line is wrong. Which line, and why?

    • Line 3, because the exons are the parts cut out
    • Line 2, because the cap and tail are added to the DNA
    • Line 1, because transcription takes place in the cytoplasm
    • Line 4, because introns are cut out in the nucleus
  11. Use this

    Notebook on strands and direction (model data), 4 rows. Columns: line, what it says. 1, The DNA opens in front of the moving polymerase and closes again behind it. 2, RNA polymerase builds the mRNA from its 5′ end toward its 3′ end. 3, The mRNA matches the coding strand, with U where the DNA has T. 4, One gene is read from both of its strands, so two mRNAs form.

  12. Pia checks a classmate’s notebook on strands and direction. Exactly one line is wrong. Which line, and why?

    • Line 2, because the mRNA is built from its 3′ end
    • Line 3, because the mRNA has the same letters as the template
    • Line 1, because the DNA stays open behind the polymerase
    • Line 4, because one strand is the template for a gene
  13. Two cells card (model data)

    cellhas a nucleus
    bacterial cellno
    plant cellyes
  14. Ren compares a bacterial cell and a plant cell. Which claim and reason are correct?

    • In the bacterial cell, a ribosome must wait for the finished mRNA, because the DNA is kept in a nucleus
    • In the plant cell, a ribosome can start reading the mRNA during its building, because transcription takes place in the cytoplasm
    • In the plant cell, a ribosome reads the pre-mRNA with its introns, because splicing is not needed
    • In the bacterial cell, a ribosome can start reading the mRNA during its building, because there is no nucleus
  15. Model template (not to scale). Zee predicts the coding strand and the mRNA for this template. Which claim and reason are correct?

    • Coding strand GTACCT and mRNA GUACCU, because both copy the template
    • Coding strand CATGGA and mRNA CATGGA, because RNA keeps T like DNA
    • Coding strand CATGGA and mRNA CAUGGA, because U replaces T
    • Coding strand CATGGA and mRNA GUACCU, because the mRNA pairs with it
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9th grade Transcription: from DNA to messenger RNA worksheet – printable
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About this worksheet

Transcription: from DNA to messenger RNA. Every version has 10 questions, and the answer key prints on the second page.

What your child will learn

  • I can say the flow of information is DNA to RNA to protein, and that this pack covers the first arrow.
  • I can say how RNA differs from DNA: its sugar is ribose, it has U where DNA has T, and it is usually one strand.
  • I can say RNA polymerase reads one DNA strand (the template strand) and builds the mRNA by pairing bases: T with A, A with U, C with G, G with C.
  • I can write the mRNA under a template strand, and tell that the mRNA matches the coding strand with U in place of T.
  • I can work backward from an mRNA to its template, and from a template to its coding strand.
  • I can order the steps: the polymerase binds at the promoter, the DNA opens, the mRNA is built, and a termination signal ends the job.

Standard: HS-LS1-1

Words to know

transcription: Copying a gene from DNA into a strand of mRNA

mRNA: Messenger RNA, the strand that carries a gene's message out of the nucleus

RNA polymerase: The enzyme that moves along DNA and builds the mRNA

template strand: The DNA strand that RNA polymerase reads for a gene

coding strand: The other DNA strand; the mRNA matches it, with U where it has T

uracil (U): The RNA base that takes the place of T

ribose: The sugar in RNA

promoter: A DNA sequence where the polymerase binds to start transcription

Common mistakes

Your child put T in an RNA strand. RNA has U where DNA has T, so its bases are A, U, G and C. Ask your child which base RNA has that DNA does not.

Your child copied the template letters into the mRNA. RNA polymerase pairs each template base with a partner base, so the letters change. Ask your child what pairs with A, T, C and G.

Your child paired the mRNA with the coding strand. The mRNA pairs with the template strand and matches the coding strand, with U in place of T. Ask your child which DNA strand RNA polymerase reads.

Questions parents ask

What is the most common mistake with transcription: from dna to messenger rna?

Your child put T in an RNA strand. RNA has U where DNA has T, so its bases are A, U, G and C. Ask your child which base RNA has that DNA does not.

Are these 9th grade science worksheets free to print?

Yes. All 6 printable versions at each level are free to print, with the answer key, and no account is needed.

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