Transcription: from DNA to messenger RNA
Grade 9 Science Worksheet - Building Confidence

Circle the best answer.
Bo reads a model of transcription. Enzyme X moves along the DNA and builds the mRNA. What is enzyme X?
- RNA polymerase, the enzyme that builds mRNA on both DNA strands at once
- DNA polymerase, the enzyme that builds a new DNA strand
- A ribosome, the structure that joins amino acids
- RNA polymerase, the enzyme that builds mRNA on one DNA strand
“not to scale” Esme reads a model of a gene in a cell with a nucleus. The mRNA is built inside compartment Z. What is Z?
Model gene in a cell with a nucleus (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: T A C G G T. mRNA, 5′ end on the left and 3′ end on the right: A U G C C A. The DNA and the mRNA being built are both inside compartment Z. Model gene in a cell with a nucleus (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: T A C G G T. mRNA, 5′ end on the left and 3′ end on the right: A U G C C A. The DNA and the mRNA being built are both inside compartment Z. compartment Z cell DNA template strand 3' end T A C G G T 5' end mRNA 5' end A U G C C A 3' end - The nucleus, the compartment that holds the DNA
- The cytoplasm, the compartment where the ribosomes are
- A ribosome, the structure that builds proteins
- A nuclear pore, the opening in the nuclear envelope
Read this first
Use this
Model strands (not to scale).
Jun fills in the blank in the mRNA built from this template. Which base goes in the blank?
Model strands (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: C T A G C T. mRNA, 5′ end on the left and 3′ end on the right: G A ? C G A. The ? is base 3 of the mRNA. Model strands (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: C T A G C T. mRNA, 5′ end on the left and 3′ end on the right: G A ? C G A. The ? is base 3 of the mRNA. DNA template strand 3' end 5' end C T A G C T mRNA 5' end 3' end G A ? C G A base 3 - T, thymine
- U, uracil
- G, guanine
- A, adenine
Kira fills in the blank in the mRNA built from this template. Which base goes in the blank?
Model strands (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: A T G C T C. mRNA, 5′ end on the left and 3′ end on the right: U ? C G A G. The ? is base 2 of the mRNA. Model strands (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: A T G C T C. mRNA, 5′ end on the left and 3′ end on the right: U ? C G A G. The ? is base 2 of the mRNA. DNA template strand 3' end 5' end A T G C T C mRNA 5' end 3' end U ? C G A G base 2 - U, uracil
- A, adenine
- G, guanine
- T, thymine
Luz fills in the blank in the mRNA built from this template. Which base goes in the blank?
Model strands (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: T A C G G T. mRNA, 5′ end on the left and 3′ end on the right: A U G ? C A. The ? is base 4 of the mRNA. Model strands (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: T A C G G T. mRNA, 5′ end on the left and 3′ end on the right: A U G ? C A. The ? is base 4 of the mRNA. DNA template strand 3' end 5' end T A C G G T mRNA 5' end 3' end A U G ? C A base 4 - A, adenine
- C, cytosine
- G, guanine
- U, uracil
Milo compares two mRNAs built from the same template. Which case is correct, and why?
Model strands (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: A C C T G A. mRNA in case P, 5′ end on the left and 3′ end on the right: U G G A C U. mRNA in case Q, 5′ end on the left and 3′ end on the right: T G G A C T. Model strands (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: A C C T G A. mRNA in case P, 5′ end on the left and 3′ end on the right: U G G A C U. mRNA in case Q, 5′ end on the left and 3′ end on the right: T G G A C T. DNA template strand 3' end 5' end A C C T G A mRNA in case P 5' end 3' end U G G A C U mRNA in case Q 5' end 3' end T G G A C T - Case Q, because RNA is made from the same letters as DNA
- Case P, because RNA has U where DNA has T
- Case P, because the mRNA copies the template letters
- Case Q, because mRNA keeps T the way DNA does
Model gene (not to scale). Noah compares two cases at one gene. Which case is right, and why?
Model gene (not to scale). DNA strand 1, 5′ end on the left and 3′ end on the right: A T G C C A. DNA strand 2, 3′ end on the left and 5′ end on the right: T A C G G T. mRNA in case P, 5′ end on the left and 3′ end on the right: A U G C C A. Case P: RNA polymerase builds one mRNA, on strand 2. Case Q: RNA polymerase builds two mRNAs, one on each strand. Model gene (not to scale). DNA strand 1, 5′ end on the left and 3′ end on the right: A T G C C A. DNA strand 2, 3′ end on the left and 5′ end on the right: T A C G G T. mRNA in case P, 5′ end on the left and 3′ end on the right: A U G C C A. Case P: RNA polymerase builds one mRNA, on strand 2. Case Q: RNA polymerase builds two mRNAs, one on each strand. Case P Case Q DNA strand 1 DNA strand 2 5' end 3' end 3' end 5' end A T G C C A T A C G G T RNA polymerase mRNA 5' end 3' end A U G C C A DNA strand 1 DNA strand 2 RNA polymerase mRNA mRNA - Case P, because the mRNA is built on the coding strand
- Case Q, because both strands of a gene are templates
- Case P, because RNA polymerase needs no template
- Case P, because one strand is the template for a gene
Pia compares two cases in a cell with a nucleus. Which case is right, and why?
Model gene in a cell with a nucleus (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: T A C G G T. mRNA, 5′ end on the left and 3′ end on the right: A U G C C A. Case P: the mRNA is built in the nucleus, then leaves it. Case Q: the mRNA is built in the cytoplasm. Model gene in a cell with a nucleus (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: T A C G G T. mRNA, 5′ end on the left and 3′ end on the right: A U G C C A. Case P: the mRNA is built in the nucleus, then leaves it. Case Q: the mRNA is built in the cytoplasm. cell nucleus cytoplasm DNA template strand 3' end 5' end T A C G G T Case P mRNA 5' end 3' end A U G C C A leaves nucleus Case Q mRNA 5' end A U G C C A 3' end - Case P, because the mRNA stays in the nucleus
- Case Q, because the DNA moves out to the cytoplasm
- Case Q, because the ribosomes are in the cytoplasm
- Case P, because the DNA stays in the nucleus
Use this
Model strands (not to scale). DNA template strand, 3′ end on the left and 5′ end on the right: T A C G G T. mRNA, 5′ end on the left and 3′ end on the right: A U G C C A. The U is base 2 of the mRNA.
Tala reads a model. Which template base sits across from the U in the mRNA?
- C, cytosine
- A, adenine
- U, uracil
- T, thymine
Use this
DNA and RNA card (model data), 3 rows. Columns: feature, DNA, RNA. sugar, deoxyribose, ribose. bases, A T C G, A U C G. strands, two, usually one.
Zee reads a card that compares DNA and RNA. Which base is found in RNA but not in DNA?
- U, uracil
- A, adenine
- C, cytosine
- T, thymine

